Rapid cloning of disease-resistance genes in plants using mutagenesis and sequence capture

Steuernagel, Burkhard, Periyannan, Sambasivam K., Hernandez-Pinzon, Inmaculada, Witek, Kamil, Rouse, Matthew N., Yu, Guotai, Hatta, Asyraf, Ayliffe, Mick, Bariana, Harbans, Jones, Jonathan D. G., Lagudah, Evans S. and Wulff, Brande (2016) Rapid cloning of disease-resistance genes in plants using mutagenesis and sequence capture. Nature Biotechnology, 34 (6). 652–655. ISSN 1087-0156

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Abstract

Wild relatives of domesticated crop species harbor multiple, diverse, disease resistance (R) genes that could be used to engineer sustainable disease control. However, breeding R genes into crop lines often requires long breeding timelines of 5–15 years to break linkage between R genes and deleterious alleles (linkage drag). Further, when R genes are bred one at a time into crop lines, the protection that they confer is often overcome within a few seasons by pathogen evolution1. If several cloned R genes were available, it would be possible to pyramid R genes2 in a crop, which might provide more durable resistance1. We describe a three-step method (MutRenSeq)-that combines chemical mutagenesis with exome capture and sequencing for rapid R gene cloning. We applied MutRenSeq to clone stem rust resistance genes Sr22 and Sr45 from hexaploid bread wheat. MutRenSeq can be applied to other commercially relevant crops and their relatives, including, for example, pea, bean, barley, oat, rye, rice and maize.

Item Type:	Article
Uncontrolled Keywords:	biotic,data integration,plant breeding
Faculty \ School:	Faculty of Science > The Sainsbury Laboratory Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Plant Sciences
Depositing User:	Pure Connector
Date Deposited:	27 Apr 2016 15:01
Last Modified:	24 Sep 2024 11:35
URI:	https://ueaeprints.uea.ac.uk/id/eprint/58441
DOI:	10.1038/nbt.3543

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