Rapid cloning of disease-resistance genes in plants using mutagenesis and sequence capture

Steuernagel, Burkhard, Periyannan, Sambasivam K., Hernandez-Pinzon, Inmaculada, Witek, Kamil, Rouse, Matthew N., Yu, Guotai, Hatta, Asyraf, Ayliffe, Mick, Bariana, Harbans, Jones, Jonathan D. G., Lagudah, Evans S. and Wulff, Brande (2016) Rapid cloning of disease-resistance genes in plants using mutagenesis and sequence capture. Nature Biotechnology, 34 (6). 652–655. ISSN 1087-0156

[img] Microsoft Word (Integrated_Supp_Fig_template_NBT_1455053544_2) - Draft Version
Download (844kB)
    [img] Microsoft Word (NBT_TR37477A_Wulff_TEXT_edited_1455053544_BS5_changes_accepted) - Submitted Version
    Download (39kB)

      Abstract

      Wild relatives of domesticated crop species harbor multiple, diverse, disease resistance (R) genes that could be used to engineer sustainable disease control. However, breeding R genes into crop lines often requires long breeding timelines of 5–15 years to break linkage between R genes and deleterious alleles (linkage drag). Further, when R genes are bred one at a time into crop lines, the protection that they confer is often overcome within a few seasons by pathogen evolution1. If several cloned R genes were available, it would be possible to pyramid R genes2 in a crop, which might provide more durable resistance1. We describe a three-step method (MutRenSeq)-that combines chemical mutagenesis with exome capture and sequencing for rapid R gene cloning. We applied MutRenSeq to clone stem rust resistance genes Sr22 and Sr45 from hexaploid bread wheat. MutRenSeq can be applied to other commercially relevant crops and their relatives, including, for example, pea, bean, barley, oat, rye, rice and maize.

      Item Type: Article
      Uncontrolled Keywords: biotic,data integration,plant breeding
      Faculty \ School: ?? TSL ??
      Faculty of Science > School of Biological Sciences
      Depositing User: Pure Connector
      Date Deposited: 27 Apr 2016 16:01
      Last Modified: 27 Mar 2019 01:02
      URI: https://ueaeprints.uea.ac.uk/id/eprint/58441
      DOI: 10.1038/nbt.3543

      Actions (login required)

      View Item