Creskey, Marybeth, Silva Angulo, Fabiola, Wu, Qing, Tamming, Levi, Fekete, Emily E. F., Cheng, Kai, Ning, Zhibin, Wang, Angela, Brito Rodrigues, Patrícia, de Rezende Rodovalho, Vinícius, Ramirez Vinolo, Marco Aurélio, Figeys, Daniel, Li, Xuguang, Trottein, Francois and Zhang, Xu (2025) Metaproteomics reveals age-specific alterations of gut microbiome in hamsters with SARS-CoV-2 infection. Gut Microbes, 17 (1). ISSN 1949-0976
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Abstract
The gut microbiome’s pivotal role in health and disease is well established. SARS-CoV-2 infection often causes gastrointestinal symptoms and is associated with changes of the microbiome in both human and animal studies. While hamsters serve as important animal models for coronavirus research, there exists a notable void in the functional characterization of their microbiomes with metaproteomics. In this study, we present a workflow for analyzing the hamster gut microbiome, including a metagenomics-derived hamster gut microbial protein database and a data-independent acquisition metaproteomics method. Using this workflow, we identified 32,419 protein groups from the fecal microbiomes of young and old hamsters infected with SARS-CoV-2. We showed age-specific changes in the expressions of microbiome functions and host proteins associated with microbiomes, providing further functional insight into the interactions between the microbiome and host in SARS-CoV-2 infection. Altogether, this study established and demonstrated the capability of metaproteomics for the study of hamster microbiomes.
Item Type: | Article |
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Uncontrolled Keywords: | covid-19,data-independent acquisition,gut microbiome,metaproteomics,microbiology,microbiology (medical),gastroenterology,infectious diseases,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2400/2404 |
Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
Related URLs: | |
Depositing User: | LivePure Connector |
Date Deposited: | 23 Jul 2025 14:30 |
Last Modified: | 28 Jul 2025 08:33 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/99983 |
DOI: | 10.1080/19490976.2025.2505117 |
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