Enzyme-cargo encapsulation peptides bind between tessellating tiles of the bacterial microcompartment shell

Gu, Shuang, Bradley-Clarke, Jack, Rose, Ruth Sarah, Warren, Martin J. and Pickersgill, Richard W. (2024) Enzyme-cargo encapsulation peptides bind between tessellating tiles of the bacterial microcompartment shell. Journal of Biological Chemistry, 300 (6). ISSN 0021-9258

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Abstract

Bacterial microcompartments are prokaryotic organelles comprising encapsulated enzymes within a thin protein shell. They facilitate metabolic processing including propanediol, choline, glycerol, and ethanolamine utilization, and they accelerate carbon fixation in cyanobacteria. Enzymes targeted to the inside of the microcompartment frequently possess a cargo-encapsulation peptide, but the site to which the peptide binds is unclear. We provide evidence that the encapsulation peptides bind to the hydrophobic groove formed between tessellating subunits of the shell proteins. In silico docking studies provide a compelling model of peptide binding to this prominent hydrophobic groove. This result is consistent with the now widely accepted view that the convex side of the shell oligomers faces the lumen of the microcompartment. The binding of the encapsulation peptide to the groove between tessellating shell protein tiles explains why it has been difficult to define the peptide binding site using other methods, provides a mechanism by which encapsulation-peptide bearing enzymes can promote shell assembly, and explains how the presence of cargo affects the size and shape of the bacterial microcompartment. This knowledge may be exploited in engineering microcompartments or disease prevention by hampering cargo encapsulation.

Item Type:	Article
Uncontrolled Keywords:	bacterial metabolism,cell compartmentalization,enzyme catalysis,peptide interaction,protein self-assembly,biochemistry,molecular biology,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1303
Faculty \ School:	Faculty of Science
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	22 Jul 2025 13:30
Last Modified:	23 Jul 2025 00:38
URI:	https://ueaeprints.uea.ac.uk/id/eprint/99966
DOI:	10.1016/j.jbc.2024.107357

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