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ToolsKühlmann, Ulrike C., Moore, Geoffrey R., James, Richard, Kleanthous, Colin and Hemmings, Andrew M. (1999) Structural parsimony in endonuclease active sites:Should the number of homing endonuclease families be redefined? FEBS Letters, 463 (1-2). pp. 1-2. ISSN 0014-5793
Full text not available from this repository.Abstract
Homing endonucleases are classified into four families based on active site sequence motifs. Through structural comparisons we have found structural similarities between the endonuclease domain of colicin E9, an H-N-H motif- containing enzyme, and both the non-specific nuclease from Serratia and I- PpoI, a His-Cys box-containing homing endonuclease. Our comparison identifies conservation at the heart of all three enzyme active sites and so argues for a re-classification of H-N-H and His-Cys box homing endonucleases as a single family. We suggest the 'ββα-Me family' of homing enzymes to reflect the three elements of secondary structure and the metal ion that define the motif.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | colicin e9,homing endonuclease,nuclease active site motif,biophysics,structural biology,biochemistry,molecular biology,genetics,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1304 |
| Faculty \ School: | Faculty of Science > School of Chemical Sciences and Pharmacy (former - to 2009) Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 13 Feb 2025 17:30 |
| Last Modified: | 16 Feb 2025 06:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/98475 |
| DOI: | 10.1016/S0014-5793(99)01499-4 |
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