Crystallization of the cytotoxic domain of a ribosome-inactivating colicin in complex with its immunity protein

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Carr, S. ORCID: https://orcid.org/0000-0001-7301-8832, Walker, D., James, R., Kleanthous, C. and Hemmings, A. M. ORCID: https://orcid.org/0000-0003-3053-3134 (2000) Crystallization of the cytotoxic domain of a ribosome-inactivating colicin in complex with its immunity protein. Acta Crystallographica Section D: Biological Crystallography, 56 (12). pp. 1630-1633. ISSN 0907-4449

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Abstract

The complex between the ribonuclease domain of the ribosome-inactivating colicin E3 and its protein inhibitor, the cognate immunity Im3, has been crystallized and preliminary X-ray characterization has been performed. Single crystals of the 1:1 complex were grown from hanging-drop vapour-diffusion experiments using 2-propanol as a precipitant. The space group is P3121 or P3221, with unit-cell parameters a = b = 93.7, c = 76.2 Å. When cryocooled, these crystals diffract to a resolution of 2.4 Å. A search for suitable conventional heavy-atom derivatives was unsuccessful and so Im3 mutants containing engineered cysteine or methionine residues have been produced for mercury soaks and selenomethionine-labelling experiments, respectively.

Item Type: Article
Uncontrolled Keywords: structural biology ,/dk/atira/pure/subjectarea/asjc/1300/1315
Faculty \ School: Faculty of Science > School of Chemical Sciences and Pharmacy (former - to 2009)
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Depositing User: LivePure Connector
Date Deposited: 21 Jan 2025 01:04
Last Modified: 21 Jan 2025 01:04
URI: https://ueaeprints.uea.ac.uk/id/eprint/98279
DOI: 10.1107/S0907444900010726

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