Biomethane is produced by acetate cleavage, not direct interspecies electron transfer: Genome-centric view and carbon isotope

Liu, Jian, Yu, Jiafeng, Tan, Yang, Run Dang, Zhou, Meng, Hernández, Marcela ORCID: https://orcid.org/0000-0002-1041-785X, Lichtfouse, Eric and Xiao, Leilei (2023) Biomethane is produced by acetate cleavage, not direct interspecies electron transfer: Genome-centric view and carbon isotope. Bioresource Technology, 387. ISSN 0960-8524

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Abstract

Understanding the source of methane (CH4) is of great significance for improving the anaerobic fermentation efficiency in bioengineering, and for mitigating the emission potential of natural ecosystems. Microbes involved in the process named direct interspecies electron transfer coupling with CO2 reduction, i.e., electrons released from electroactive bacteria to reduce CO2 into CH4, have attracted considerable attention for wastewater treatment in the past decade. However, how the synergistic effect of microbiota contributes to this anaerobic carbon metabolism accompanied by CH4 production still remains poorly understood, especial for wastewater with antibiotic exposure. Results show that enhancing lower-abundant acetoclastic methanogens and acetogenic bacteria, rather than electroactive bacteria, contributed to CH4 production, based on a metagenome-assembled genomes network analysis. Natural and artificial isotope tracing of CH4 further confirmed that CH4 mainly originated from acetoclastic methanogenesis. These findings reveal the contribution of direct acetate cleavage (acetoclastic methanogenesis) and provide insightsfor further regulation of methanogenic strategies.

Item Type:	Article
Additional Information:	Funding Information: This work was supported by Youth Innovation Promotion Association, CAS (2021213), the National Natural Science Foundation of China (42077025, 42277236), the Youth Science and Technology Innovation Plan of Universities in Shandong (2019KJE007). All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. The metagenomic sequencing data have been deposited in the National Center for Biotechnology Information (NCBI) Short Read Archive database (SRA, https://www.ncbi.nlm.nih.gov/bioproject/PRJNA734822). Funding Information: This work was supported by Youth Innovation Promotion Association, CAS (2021213), the National Natural Science Foundation of China (42077025, 42277236), the Youth Science and Technology Innovation Plan of Universities in Shandong (2019KJE007). Publisher Copyright: © 2023 Elsevier Ltd
Uncontrolled Keywords:	acetate metabolism,anaerobic digestion,antibiotic exposure,metagenome-assembled genome,bioengineering,waste management and disposal,environmental engineering,renewable energy, sustainability and the environment ,/dk/atira/pure/subjectarea/asjc/1500/1502
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Molecular Microbiology
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	21 Nov 2024 15:30
Last Modified:	27 Nov 2024 10:45
URI:	https://ueaeprints.uea.ac.uk/id/eprint/97744
DOI:	10.1016/j.biortech.2023.129589

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