Anti-melanogenic potential of Malabar spinach (Basella Alba) in human melanoma cells with oxidative stress suppression and anti-inflammatory activities

Linsaenkart, Pichchapa, Ruksiriwanich, Warintorn, Sringarm, Korawan, Arjin, Chaiwat, Rachtanapun, Pornchai, Chittasupho, Chuda, Castagnini, Juan M., Chutoprapat, Romchat, Mueller, Anja ORCID: https://orcid.org/0000-0003-0774-0434 and Boonpisuttinant, Korawinwich (2024) Anti-melanogenic potential of Malabar spinach (Basella Alba) in human melanoma cells with oxidative stress suppression and anti-inflammatory activities. Foods, 13 (18). ISSN 2304-8158

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Abstract

Basella alba has been used in Thai remedies to treat skin disorders, but scientific evidence supporting its efficacy is currently limited. In this study, we investigated the inhibitory effects of B. alba extracts on melanin production using melanoma cells, as well as their impact on oxidative stress and inflammation in keratinocytes. The results demonstrate that B. alba extracts inhibited melanin content and cellular tyrosinase activity in 3-isobutyl-1-methylxanthine (IBMX)-induced melanoma cells by downregulating MITF and the pigmentary genes TYR, TRP-1, and DCT. Interestingly, the MITF regulator gene was inhibited by both the 50% and 95% ethanolic extracts of B. alba with levels of 0.97 ± 0.19 and 0.92 ± 0.09 of the control, respectively, which are comparable to those observed in the arbutin treatment group at 0.84 ± 0.05 of the control. Moreover, after hydrogen peroxide (H 2O 2) exposure, pretreatment with B. alba reduced lipid peroxidation byproducts and increased the levels of antioxidant-related genes, including SOD-1, GPX-1, and NRF2. Notably, the suppression of the POMC promoter gene in keratinocytes was observed, which may disrupt melanogenesis in melanocytes involving the MC1R signaling pathway. MC1R mRNA expression decreased in the treatments with 50% and 95% ethanolic extracts of B. alba, with relative levels of 0.97 ± 0.18 and 0.90 ± 0.10 of the control, respectively, similar to the arbutin-treated group (0.88 ± 0.25 of control). A significant reduction in nitric oxide was also observed in the B. alba-treated groups, along with a decrease in genes associated with pro-inflammatory cytokines, including IL-1β, IL-6, and COX-2. These findings suggest that B. alba has potential in the prevention of skin-related problems.

Item Type:	Article
Additional Information:	Data Availability Statement: The original contributions presented in the study are included in the article; further inquiries can be directed to the corresponding author. Funding information: This research was funded by the CMU Presidential Scholarship (funding number: 2564-017), the Thailand Research Fund (TRF) Research Team Promotion Grant, RTA, Senior Re-search Scholar (N42A671052); the Center of Excellence in Agro-Bio-Circular-Green Industry (Agro-BCG), Faculty of Agro-Industry, Chiang Mai University (CoE/RG66/67-P001); the International Relations Division, Office of the University, Chiang Mai University, and partially supported by Chiang Mai University.
Uncontrolled Keywords:	basella alba,anti-inflammation,anti-melanogenesis,antioxidant,caffeic acid,catechin,p-coumaric acid,food science,microbiology,health(social science),health professions (miscellaneous),plant science ,/dk/atira/pure/subjectarea/asjc/1100/1106
Faculty \ School:	Faculty of Science > School of Chemistry, Pharmacy and Pharmacology
UEA Research Groups:	Faculty of Science > Research Groups > Molecular and Tissue Pharmacology
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	18 Sep 2024 13:30
Last Modified:	12 Oct 2024 00:00
URI:	https://ueaeprints.uea.ac.uk/id/eprint/96764
DOI:	10.3390/foods13182943

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