A comparison by magnetic circular dichroism of compound X and compound II of horseradish peroxidase

Foote, Nicholas, Gadsby, Paul M. A., Field, Robert A. ORCID: https://orcid.org/0000-0001-8574-0275, Greenwood, Colin and Thomson, Andrew J. (1987) A comparison by magnetic circular dichroism of compound X and compound II of horseradish peroxidase. FEBS Letters, 214 (2). pp. 347-350. ISSN 0014-5793

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Abstract

The chlorite product of horseradish peroxidase, compound X, is shown by magnetic circular dichroism (MCD) spectroscopy in the temperature range 1.6-50 K to have a very similar haem structure to compound II under the same conditions (pH 10.7). Both are concluded to contain the Fe(IV)=0 group. The MCD spectrum also detects an unusual species, absorbing at wavelengths between 600 and 750 nm, that has magnetic properties different from those of the ferryl haem group. It is suggested that this is a species at the same oxidation level as ferryl haem but with the porphyrin ring having suffered a one-electron oxidation, i.e. [Fe(III) P.+].

Item Type:	Article
Additional Information:	Funding Information: A.J.T. and C.G. thank the SERC and the Royal Society for grants in support of this work.
Uncontrolled Keywords:	compound ii,compound x,horseradish peroxidase,magnetic circular dichroism,biophysics,structural biology,biochemistry,molecular biology,genetics,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1304
Faculty \ School:	Faculty of Science > School of Chemical Sciences and Pharmacy (former - to 2009) Faculty of Science > School of Biological Sciences
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	11 Sep 2024 14:30
Last Modified:	25 Sep 2024 18:09
URI:	https://ueaeprints.uea.ac.uk/id/eprint/96731
DOI:	10.1016/0014-5793(87)80085-6

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