Conservation of the PBL-RBOH immune module in land plants

Chu, Jiashu, Monte, Isabel, DeFalco, Thomas A., Köster, Philipp, Derbyshire, Paul, Menke, Frank ORCID: https://orcid.org/0000-0003-2490-4824 and Zipfel, Cyril (2023) Conservation of the PBL-RBOH immune module in land plants. Current Biology, 33 (6). 1130-1137.e5. ISSN 0960-9822

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Abstract

The rapid production of reactive oxygen species (ROS) is a key signaling output in plant immunity. In the angiosperm model species Arabidopsis thaliana (hereafter Arabidopsis), recognition of non- or altered-self elicitor patterns by cell-surface immune receptors activates the receptor-like cytoplasmic kinases (RLCKs) of the AVRPPHB SUSCEPTIBLE 1 (PBS1)-like (PBL) family, particularly BOTRYTIS-INDUCED KINASE1 (BIK1).1,2,3 BIK1/PBLs in turn phosphorylate the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) to induce apoplastic ROS production.4,5 PBL and RBOH functions in plant immunity have been extensively characterized in flowering plants. Much less is known about the conservation of pattern-triggered ROS signaling pathways in non-flowering plants. In this study, we show that in the liverwort Marchantia polymorpha (hereafter Marchantia), single members of the RBOH and PBL families, namely MpRBOH1 and MpPBLa, are required for chitin-induced ROS production. MpPBLa directly interacts with and phosphorylates MpRBOH1 at specific, conserved sites within its cytosolic N terminus, and this phosphorylation is essential for chitin-induced MpRBOH1-mediated ROS production. Collectively, our work reveals the functional conservation of the PBL-RBOH module that controls pattern-triggered ROS production in land plants.

Item Type:	Article
Additional Information:	Funding Information: I.M. was supported by a long-term fellowship from the Human Frontier Science Program (LT000274/2019-L). T.A.D. was supported by postdoctoral fellowships from the European Molecular Biology Organization (#100-2017) and the Natural Sciences and Engineering Research Council of Canada (NSERC PDF-532561-2019). P.K. was supported by the EU Horizon 2020 Research and Innovation Program, Marie Skłodowska-Curie Actions (grant agreement no. 892398). This research was supported by the University of Zurich (C.Z.) and the Swiss National Science Foundation (grant agreement no. 31003A_182625 to C.Z.), and the Gatsby Charitable Foundation.
Uncontrolled Keywords:	marchantia,nadph oxidase,plant immunity,evolution,kinase,phosphorylation,reactive oxygen species,signaling,neuroscience(all),biochemistry, genetics and molecular biology(all),agricultural and biological sciences(all) ,/dk/atira/pure/subjectarea/asjc/2800
Faculty \ School:	Faculty of Science > The Sainsbury Laboratory Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Plant Sciences
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	24 Feb 2023 16:30
Last Modified:	27 Mar 2023 08:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/91267
DOI:	10.1016/j.cub.2023.01.050

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