Dobbin, Paul S., Butt, Julea N. ORCID: https://orcid.org/0000-0002-9624-5226, Powell, Anne K., Reid, Graeme A. and Richardson, David J.
ORCID: https://orcid.org/0000-0002-6847-1832
(1999)
Characterization of a flavocytochrome that is induced during the anaerobic respiration of Fe3+ by Shewanella frigidimarina NCIMB400.
Biochemical Journal, 342 (2).
pp. 439-448.
ISSN 0264-6021
Abstract
A 63.9 kDa periplasmic tetrahaem flavocytochrome c3, designated Ifc3, was found to be expressed in Shewanella frigidimarina NCIMB400 grown anaerobically with ferric citrate or ferric pyrophosphate as the sole terminal electron acceptor, but not in anaerobic cultures of the bacterium with other respiratory substrates. Ifc3 was purified to homogeneity and revealed by biochemical, spectroscopic and primary structure analyses to contain four low-spin bis-His-ligated c3-haems, with midpoint reduction potentials of -73, -141, -174 and -259 mV. A low-potential flavin was present in the form of non-covalently bound FAD; the protein possessed a unidirectional fumarate reductase activity. Disruption of the chromosomal gene encoding Ifc3, ifcA, did not lead to a significant change in the rate of Fe3+ reduction in batch culture. However, during such growth the Ifc3-deficient mutant produced both a 35 kDa periplasmic c-type cytochrome and a 45 kDa membrane-associated c-type cytochrome at markedly higher levels than did the parent strain. Nucleotide sequencing data from directly upstream of ifcA indicated the presence of an open reading frame encoding a putative outer-membrane β-barrel protein of 324 amino acid residues.
Item Type: | Article |
---|---|
Uncontrolled Keywords: | bacteria,ferric reductase,flavin,haem,iron,biochemistry,molecular biology,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1303 |
Faculty \ School: | Faculty of Science > School of Chemical Sciences and Pharmacy (former - to 2009) Faculty of Science > School of Biological Sciences |
UEA Research Groups: | Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry |
Related URLs: | |
Depositing User: | LivePure Connector |
Date Deposited: | 14 Jul 2022 16:31 |
Last Modified: | 15 May 2023 00:54 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/86129 |
DOI: | 10.1042/0264-6021:3420439 |
Actions (login required)
![]() |
View Item |