Bioavailability and mechanisms of iron uptake from pea ferritin and ferric phosphate nanoparticles using cell culture models

Perfecto Jr, Antonio (2017) Bioavailability and mechanisms of iron uptake from pea ferritin and ferric phosphate nanoparticles using cell culture models. Doctoral thesis, University of East Anglia.

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Abstract

Iron deficiency is a global health burden. Despite sustained efforts to eradicate iron deficiency, it remains the number one nutritional deficiency in the world, affecting over two billion people [1]. One of the main causes of iron deficiency is insufficient dietary intake and/or low bioavailability. Public health approaches to eradicate iron deficiency include supplementation and food fortification. The aims of my PhD project were to investigate the bioavailability and mechanisms of apical uptake of novel iron forms using cell culture models and thereby provide insight toward improving iron deficiency.
Chapter 1 is an introduction to the importance of iron to human health, strategies to alleviate iron deficiency, our current understanding of dietary intestinal iron absorption, and methods for measuring iron bioavailability, with a particular emphasis on the in vitro digestion / Caco-2 cell model. Chapter 2 provides an outline of the basic methods used to study iron uptake in Caco-2 and Hutu-80 cells. Chapter 3 investigates the use of ferric phosphate nanoparticles (NP-FePO4) as a source of iron fortification. NP-FePO4 was kindly donated by Michael Zimmerman�s group based at ETH Zurich. Chapter 4 focuses on the use of plant-derived ferritin (phytoferritin) as a potential iron supplement. Phytoferritin was extracted and purified from marrowfat peas by Janneke Balk�s lab group, based at the John Innes Centre. Chapter 5 examines the use of the commercially available iron ingot, Lucky Iron Fish©, as a source of home iron fortification. Chapters 3, 4, and 5 are the basis of 3 separately submitted manuscripts; one of which has been accepted, and the other two currently under peer review. Lastly, a general discussion of the experiments undertaken and recommendations for future work are outlined in Chapter 6.
All three iron sources used in this thesis comprise the newest strategies for iron therapeutics. By assessing iron bioavailability, and more importantly the mechanisms of iron uptake, it is hoped that the work will prove insightful and can be used in the design of future prospective human trials.

Item Type:	Thesis (Doctoral)
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User:	Bruce Beckett
Date Deposited:	26 Jul 2018 10:33
Last Modified:	31 Dec 2020 01:38
URI:	https://ueaeprints.uea.ac.uk/id/eprint/67869
DOI:

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