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Kellingray, Lee, Gall, Gwénaëlle Le 
ORCID: https://orcid.org/0000-0002-1379-2196, Defernez, Marianne, Beales, Ian L.P., Franslem-Elumogo, Ngozi and Narbad, Arjan
(2018)
Microbial taxonomic and metabolic alterations during faecal microbiota transplantation to treat Clostridium difficile infection.
Journal of Infection, 77 (2).
pp. 107-118.
ISSN 0163-4453
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Abstract
Objectives: This study aimed to examine changes to the microbiota composition and metabolic profiles of seven patients with recurrent Clostridium difficile infection (rCDI), following treatment with faecal microbiota transplant (FMT). Methods: 16S rDNA sequencing and 1H NMR were performed on faecal samples from the patients (pre-, post-FMT, and follow-up) and the associated donor samples. Sparse partial-least-square analysis was used to identify correlations between the two datasets. Results: The patients’ microbiota post-FMT tended to shift towards the donor microbiota, specifically through proportional increases of Bacteroides, Blautia, and Ruminococcus, and proportional decreases of Enterococcus, Escherichia, and Klebsiella. However, although cured of infection, one patient, who suffers from chronic alcohol abuse, retained the compositional characteristics of the pre-FMT microbiota. Following FMT, increased levels of short-chain fatty acids, particularly butyrate and acetate, were observed in all patients. Sparse partial-least-square analysis confirmed a positive correlation between butyrate and Bacteroides, Blautia, and Ruminococcus, with a negative correlation between butyrate and Klebsiella and Enterococcus. Conclusions: Clear differences were observed in the microbiota composition and metabolic profiles between donors and rCDI patients, which were largely resolved in patients following FMT. Increased levels of butyrate appear to be a factor associated with resolution of rCDI.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | clostridium difficile,faecal microbiota transplantation,metataxonomics,metabonomics,alcohol abuse,sparse partial-least-square analysis |
| Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Science > School of Pharmacy Faculty of Science > School of Chemistry Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Lifespan Health Faculty of Medicine and Health Sciences > Research Centres > Population Health |
| Related URLs: | |
| Depositing User: | Pure Connector |
| Date Deposited: | 09 May 2018 11:30 |
| Last Modified: | 06 Jun 2024 15:02 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/66974 |
| DOI: | 10.1016/j.jinf.2018.04.012 |
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