Wheat vacuolar iron transporter TaVIT2 transports Fe and Mn and is effective for biofortification

Connorton, James M., Jones, Eleanor R., Rodriguez-Ramiro, Ildefonso, Fairweather-Tait, Susan ORCID: https://orcid.org/0000-0002-1413-5569, Uauy, Cristobal and Balk, Janneke (2017) Wheat vacuolar iron transporter TaVIT2 transports Fe and Mn and is effective for biofortification. Plant Physiology, 174 (4). pp. 2434-2444. ISSN 0032-0889

Preview	PDF (Accepted manuscript) - Accepted Version Download (1MB) | Preview
Preview	PDF (Connorton_PlantPhysiology_2017_174_4_2434) - Published Version Available under License Unspecified licence. Download (1MB) | Preview

Abstract

Increasing the intrinsic nutritional quality of crops, known as biofortification, is viewed as a sustainable approach to alleviate micronutrient deficiencies. In particular iron deficiency anaemia is a major global health issue, but the iron content of staple crops such as wheat is difficult to change because of genetic complexity and homeostasis mechanisms. To identify target genes for biofortification of wheat (Triticum aestivum), we functionally characterized homologs of the Vacuolar Iron Transporter (VIT). The wheat genome contains two VIT paralogs, TaVIT1 and TaVIT2, which have different expression patterns, but are both low in the endosperm. TaVIT2, but not TaVIT1, was able to rescue growth of a yeast mutant lacking the vacuolar iron transporter. TaVIT2 also complemented a manganese transporter mutant, but not a vacuolar zinc transporter mutant. By over-expressing TaVIT2 under the control of an endosperm-specific promoter, we achieved a > 2-fold increase in iron in white flour fractions, exceeding minimum legal fortification levels in countries such as the UK. The anti-nutrient phytate was not increased and the iron in the white flour fraction was bioavailable in-vitro, suggesting that food products made from the biofortified flour could contribute to improved iron nutrition. The single-gene approach impacted minimally on plant growth and was also effective in barley. Our results show that by enhancing vacuolar iron transport in the endosperm, this essential micronutrient accumulated in this tissue bypassing existing homeostatic mechanisms.

Item Type:	Article
Additional Information:	Funding information: This work was funded by HarvestPlus (J.M.C., J.B., and C.U.) and by the BBSRC Institute Strategic Programme Grant BB/J004561/1 (C.U. and J.B.) and DRINC2 grant BB/L025515/1 (I.R.-R., S.F.-T., and J.B.).
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Musculoskeletal Medicine Faculty of Medicine and Health Sciences > Research Centres > Lifespan Health Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Groups > Plant Sciences
Depositing User:	Pure Connector
Date Deposited:	13 Jul 2017 05:05
Last Modified:	25 Sep 2024 12:51
URI:	https://ueaeprints.uea.ac.uk/id/eprint/64095
DOI:	10.1104/pp.17.00672

Downloads

Actions (login required)

View Item