Kinetic analysis of copper transfer from a chaperone to its target protein mediated by complex formation

Kay, Kristine L., Zhou, Liang, Tenori, Leonardo, Bradley, Justin Michael, Singleton, Chloe, Kihlken, Margaret A., Ciofi-Baffoni, Simone and Le Brun, Nicolas ORCID: https://orcid.org/0000-0001-9780-4061 (2017) Kinetic analysis of copper transfer from a chaperone to its target protein mediated by complex formation. Chemical Communications, 53 (8). pp. 1397-1400. ISSN 1359-7345

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Abstract

Chaperone proteins that traffic copper around the cell minimise its toxicity by maintaining it in a tightly bound form. The transfer of copper from chaperones to target proteins is promoted by complex formation, but the kinetic characteristics of transfer have yet to be demonstrated for any chaperone-target protein pair. Here we report studies of copper transfer between the Atx1-type chaperone CopZ from Bacillus subtilis and the soluble domains of its cognate P-type ATPase transporter, CopAab. Transfer of copper from CopZ to CopAab was found to occur rapidly, with a rate constant at 25 °C of ∼267 s−1, many orders of magnitude higher than that for Cu(I) dissociation from CopZ in the absence of CopAab. The data demonstrate that complex formation between CopZ and CopAab, evidence for which is provided by NMR and electrospray ionisation mass spectrometry, dramatically enhances the rate of Cu(I) dissociation from CopZ.

Item Type:	Article
Additional Information:	This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Faculty \ School:	Faculty of Science > School of Chemistry Faculty of Science > School of Chemical Sciences and Pharmacy (former - to 2009)
UEA Research Groups:	Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017) Faculty of Science > Research Groups > Centre for Ocean and Atmospheric Sciences Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Chemistry of Life Processes
Depositing User:	Pure Connector
Date Deposited:	06 Jan 2017 00:03
Last Modified:	22 Oct 2022 02:04
URI:	https://ueaeprints.uea.ac.uk/id/eprint/61957
DOI:	10.1039/C6CC08966F

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