Spin Saturation Transfer Difference NMR (SSTD NMR): a new tool to obtain kinetic parameters of chemical exchange processes

Quirós, María Teresa, Macdonald, Colin, Angulo, Jesús ORCID: https://orcid.org/0000-0001-7250-5639 and Paz, María ORCID: https://orcid.org/0000-0001-9037-349X (2016) Spin Saturation Transfer Difference NMR (SSTD NMR): a new tool to obtain kinetic parameters of chemical exchange processes. Journal of visualized experiments : JoVE (117).

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Abstract

This detailed protocol describes the new Spin Saturation Transfer Difference Nuclear Magnetic Resonance protocol (SSTD NMR), recently developed in our group to study processes of mutual-site chemical exchange that are difficult to analyze by traditional methods. As the name suggests, this method combines the Spin Saturation Transfer method used for small molecules, with the Saturation Transfer Difference (STD) NMR method employed for the study of protein-ligand interactions, by measuring transient spin saturation transfer along increasing saturation times (build-up curves) in small organic and organometallic molecules undergoing chemical exchange.

Item Type:	Article
Uncontrolled Keywords:	nmmr,spin saturation transfer,saturation transfer difference,kinetics,chemical exchange,noe,thermodynamic parameters,sstd nmr
Faculty \ School:	Faculty of Science > School of Chemistry (former - to 2024) Faculty of Science Faculty of Science > School of Pharmacy (former - to 2024)
UEA Research Groups:	Faculty of Science > Research Groups > Chemistry of Materials and Catalysis Faculty of Science > Research Groups > Pharmaceutical Materials and Soft Matter
Depositing User:	Pure Connector
Date Deposited:	24 May 2016 13:00
Last Modified:	21 Nov 2024 03:18
URI:	https://ueaeprints.uea.ac.uk/id/eprint/59041
DOI:	10.3791/54499

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