Elucidating pathways of Toxoplasma gondii invasion in the gastrointestinal tract: involvement of the tight junction protein occludin

Weight, Caroline, Jones, Emily, Horn, Nikki, Wellner, Nikolaus and Carding, Simon (2015) Elucidating pathways of Toxoplasma gondii invasion in the gastrointestinal tract: involvement of the tight junction protein occludin. Microbes and Infection, 17 (10). pp. 698-709. ISSN 1286-4579

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Abstract

Toxoplasma gondii is an obligate intracellular parasite infecting one third of the world’s population. The small intestine is the parasite’s primary route of infection, although the pathway of epithelium transmigration remains unclear. Using an in vitro invasion assay and live imaging we showed that T. gondii (RH) tachyzoites infect and transmigrate between adjacent intestinal epithelial cells in polarized monolayers without altering barrier integrity, despite eliciting the production of specific inflammatory mediators and chemokines. During invasion, T. gondii co-localized with occludin. Reducing the levels of endogenous cellular occludin with specific small interfering RNAs significantly reduced the ability of T. gondii to penetrate between and infect epithelial cells. Furthermore, an in vitro invasion and binding assays using recombinant occludin fragments established the capacity of the parasite to bind occludin and in particular to the extracellular loops of the protein. These findings provide evidence for occludin playing a role in the invasion of T. gondii in small intestinal epithelial cells.

Item Type:	Article
Uncontrolled Keywords:	toxoplasma gondii,occludin,invasion,intestinal epithelial cells
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Depositing User:	Pure Connector
Date Deposited:	01 Apr 2016 10:18
Last Modified:	02 Mar 2023 01:51
URI:	https://ueaeprints.uea.ac.uk/id/eprint/58060
DOI:	10.1016/j.micinf.2015.07.001

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