Disc-shaped polyoxyethylene glycol glycerides gel nanoparticles as novel protein delivery vehicles

Codoni, Doroty, Cowan, Jonathan, Bradley, Jenna, O'Connell, Maria ORCID: https://orcid.org/0000-0002-0267-0951, McAuley, William and Qi, Sheng ORCID: https://orcid.org/0000-0003-1872-9572 (2015) Disc-shaped polyoxyethylene glycol glycerides gel nanoparticles as novel protein delivery vehicles. International Journal of Pharmaceutics, 496 (2). pp. 1015-1025. ISSN 0378-5173

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Abstract

Disc-shaped nanoparticles with high aspect ratios have been reported to show preferential cellular uptake in vitro by mammalian cells. However, engineering and producing such disc-shaped nanoparticles are often complex. This study reports for the first time the use of a single, approved pharmaceutical excipient to prepare stable disc-shaped nanoparticles with a high aspect ratio via a simple, organic solvent free process. These disc-shaped nanoparticles were formed by fragmentation of stearoyl macrogol-32 glycerides (Gelucire 50/13) hydrogels. The nanoparticles showed good physical stability as a result of their outer coating of polyethylene glycol (PEG) that is a part of Gelucire composition. Using lysozyme as a model hydrophilic protein, these nanoparticles demonstrated a good loading capacity for hydrophilic macromolecules, mainly via surface adsorption. As a result of the higher hydrophobicity of the core of the nano-discs, the loading efficiency of hydrophobic model components, such as Coumarin-6, was significantly increased in comparison to the model hydrophilic compound. These Gelucire nano-discs exhibited no cytotoxicity at the tested level of 600µg/ml for Caco-2 cells. Rapid in vitro cellular uptake of the disc-shaped nanoparticles by Caco-2 and H292 epithelial cells was observed. This rapid internalisation was attributed to the high aspect ratio of the disc-shape nanoparticles which provides a high contact surface area between the particles and cells and may lower the strain energy required for membrane deformation during uptake. The results of this study demonstrate the excellent potential of Gelucire nano-discs as effective nanocarriers for drug delivery and which can be manufactured using a simple solvent- free process.

Item Type:	Article
Uncontrolled Keywords:	drug delivery,polyoxyethylene glycol glycerides,disc-shape nanoparticles,cellular uptake
Faculty \ School:	Faculty of Science > School of Pharmacy (former - to 2024)
UEA Research Groups:	Faculty of Science > Research Groups > Drug Delivery and Pharmaceutical Materials (former - to 2017) Faculty of Science > Research Groups > Pharmaceutical Cell Biology (former - to 2017) Faculty of Science > Research Groups > Molecular and Tissue Pharmacology Faculty of Science > Research Groups > Pharmaceutical Materials and Soft Matter
Depositing User:	Pure Connector
Date Deposited:	19 Dec 2015 07:16
Last Modified:	24 Sep 2024 11:26
URI:	https://ueaeprints.uea.ac.uk/id/eprint/55789
DOI:	10.1016/j.ijpharm.2015.10.067

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