Impact of a single oral dose of 100,000 IU vitamin D3 on profiles of serum 25(OH)D3 and its metabolites 24,25(OH)2D3, 3-epi-25(OH)D3, and 1,25(OH)2D3 in adults with vitamin D insufficiency

Saleh, Lanja, Tang, Jonathan CY, Gawinecka, Joanna, Boesch, Lukas, Fraser, William D, von Eckardstein, Arnold and Nowak, Albina (2017) Impact of a single oral dose of 100,000 IU vitamin D3 on profiles of serum 25(OH)D3 and its metabolites 24,25(OH)2D3, 3-epi-25(OH)D3, and 1,25(OH)2D3 in adults with vitamin D insufficiency. Clinical Chemistry and Laboratory Medicine, 55 (12). pp. 1912-1921. ISSN 1434-6621

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    Abstract

    We investigate the effect of a high dose of vitamin D3 on circulating concentrations of 25(OH)D3 and its metabolites 24,25(OH)2D3, 3-epi-25(OH)D3, and 1,25(OH)2D3 in healthy individuals with self-perceived fatigue and vitamin D insufficiency (25(OH)D3 <50 nmol/L). Methods: 107 study participants (age 20–50 years) were randomizedto receive a single 100,000 IU dose of vitamin D3 (n= 52) or placebo (n= 55).Vitamin D metabolite concentrations in serum were measured before, and 4 weeksafter, supplementation. Results: Overall, 52% of participants receiving vitamin D3attained a serum 25(OH)D3 level >75 nmol/L. Among individuals who receivedvitamin D3, there were significant increases in serum concentrations of25(OH)D3 and its metabolites 24,25(OH)2D3, 3-epi-25(OH)D3, and1,25(OH)2D3 at 4 weeks; however, inter-individual variability inthese changes was substantial. Positive correlations between serum 25(OH)D3 and24,25(OH)2D3 and 3-epi-25(OH)D3, and a significant negativecorrelation between serum 1,25(OH)2D3 and 3-epi-25(OH)D3, were found4 weeks after supplementation. The 24,25(OH)2D3/25(OH)D3 and24,25(OH)2D3/1,25(OH)2D3 ratios were significantly increased,compared with baseline, in participants receiving vitamin D3. Baseline 25(OH)D3concentration was the only factor predictive of the change in 25(OH)D3 aftersupplementation. Conclusions: Administration of a singlehigh dose of vitamin D3 leads to a significant increase in concentrations of25(OH)D3, 24,25(OH)2D3, 3-epi-25(OH)D3 and 1,25(OH)2D3;induction of the catabolic pathway predominates over the production of 1,25(OH)2D3.Due to the high inter-individual variation in the 25(OH)D3 response to supplementation,any given dose of vitamin D is unlikely to achieve optimal vitamin D status inall treated individuals

    Item Type: Article
    Faculty \ School: University of East Anglia > Faculty of Medicine and Health Sciences > Research Groups > Musculoskeletal Science
    Faculty of Medicine and Health Sciences > Norwich Medical School
    Depositing User: Pure Connector
    Date Deposited: 07 Feb 2017 02:40
    Last Modified: 25 Jul 2018 13:15
    URI: https://ueaeprints.uea.ac.uk/id/eprint/62334
    DOI: 10.1515/cclm-2016-1129

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