A standardised saffron extract improves subjective and objective sleep quality in healthy older adults with sleep complaints: results from the Gut-Sleep-Brain Axis randomised, double-blind, placebo-controlled pilot study

Lang, Leonie, Ditton, Annabel, Stanescu, Andi, Jainani, Vernisse, Mcarthur, Simon, Pourtau, Line, Gaudout, David, Pontifex, Matthew G., Tsigarides, Jordan, Steward, Taylor, Sami, Saber, Muller, Michael, Hornberger, Michael, Vauzour, David and Lazar, Alpar S. (2025) A standardised saffron extract improves subjective and objective sleep quality in healthy older adults with sleep complaints: results from the Gut-Sleep-Brain Axis randomised, double-blind, placebo-controlled pilot study. Food & Function. ISSN 2042-6496

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Abstract

Sleep disturbances are associated with an increased risk of neurodegenerative diseases and alterations in gut microbiota composition. Saffron (Crocus sativus) has been shown to improve sleep and modulate the gut microbiome, but its effect on sleep quality via the gut microbiota-brain axis remain largely unexplored. This randomised, placebo-controlled pilot study investigated the impact of four weeks of saffron supplementation (30mg/day) on sleep quality and gut microbiota in older adults (ages 55-85) with self-reported sleep complaints (N=52). Subjective sleep quality was assessed using validated questionnaires, while objective measures were captured via an electroencephalography-based sleep tracker. Gut microbiota composition was analysed in a subgroup (N=26). Saffron supplementation significantly improved subjective sleep quality (p=0.02) and sleep efficiency (p=0.04). Objective outcomes included reduced latency to persistent sleep (p=0.003) and shorter sleep onset latency (p=0.03). Microbiome analysis using linear discriminant analysis effect size (LEfSe) revealed significant increases in Faecalibacterium (q=0.013), Lachnoclostridium (q=0.045), Prevotella (q=0.022), UBA1819 (q=0.020) and Oscillibacter (q=0.045), alongside a decrease in Dialister (q=0.028). Univariate analysis further identified increases in Lachnospiraceae-UGC-001 (p=0.020) and Roseburia (p=0.03), with a reduction in Turicibacter (p=0.045) in the saffron group. Correlational analyses revealed that Oscillibacter and UBA1819 were positively associated with subjective sleep efficiency (r=0.63, p=0.0007) and inversely associated with sleep latency (r=-0.39, p=0.04). Alterations in in Dialister, Turicibacter and UBA1819 correlated with objective sleep quality parameters including wake duration, latency to persistent sleep and wake-after-sleep-onset. In summary, four-weeks saffron supplementation improved both subjective and objective sleep quality in older adults with sleep complaints, and modulated gut microbiota composition, particularly increasing short-chain fatty acids producing bacteria. These findings pave the way for further randomised controlled trials exploring the links between sleep quality and gut health and may help devising new preventative strategies for age-related brain disorders.

Item Type: Article
Uncontrolled Keywords: pittsburgh sleep quality index,eeg,latency to persistent sleep,sleep onset latency,gut microbiome,sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Faculty of Medicine and Health Sciences > School of Health Sciences
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine
Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Faculty of Medicine and Health Sciences > Research Centres > Mental Health and Social Care (fka Lifespan Health)
Faculty of Science > Research Groups > Norwich Epidemiology Centre
Faculty of Medicine and Health Sciences > Research Groups > Norwich Epidemiology Centre
Faculty of Medicine and Health Sciences > Research Groups > Mental Health
Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging
Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Faculty of Medicine and Health Sciences > Research Groups > Dementia & Complexity in Later Life
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Depositing User: LivePure Connector
Date Deposited: 31 Jul 2025 10:30
Last Modified: 31 Jul 2025 10:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/99995
DOI: 10.1039/D5FO00917K

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