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ToolsComerlato, Carolina Baldisserotto, Zhang, Xu, Walker, Krystal, Mayne, Janice, Figeys, Daniel and Brandelli, Adriano (2024) The Influence of Protein Secretomes of Enterococcus durans on ex vivo Human Gut Microbiome. Probiotics and Antimicrobial Proteins, 16 (6). pp. 1954-1965. ISSN 1867-1306
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Abstract
The gut microbiome plays a critical role to all animals and humans health. Methods based on ex vivo cultures are time and cost-effective solutions for rapid evaluation of probiotic effects on microbiomes. In this study, we assessed whether the protein secretome from the potential probiotic Enterococcus durans LAB18S grown on fructoligosaccharides (FOS) and galactoligosaccharides (GOS) had specific effects on ex vivo cultured intestinal microbiome obtained from a healthy individual. Metaproteomics was used to evaluate changes in microbial communities of the human intestinal microbiome. Hierarchical clustering analysis revealed 654 differentially abundant proteins from the metaproteome samples, showing that gut microbial protein expression varied on the presence of different E. durans secretomes. Increased amount of Bacteroidetes phylum was observed in treatments with secretomes from E. durans cultures on FOS, GOS and albumin, resulting in a decrease of the Firmicutes to Bacteroidetes (F/B) ratio. The most functionally abundant bacterial taxa were Roseburia, Bacteroides, Alistipes and Faecalibacterium. The results suggest that the secretome of E. durans may have favorable effects on the intestinal microbial composition, stimulating growth and different protein expression of beneficial bacteria. These findings suggest that proteins secreted by E. durans growing on FOS and GOS have different effects on the modulation of gut microbiota functional activities during cultivation.
| Item Type: | Article |
|---|---|
| Additional Information: | Publisher Copyright: © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. |
| Uncontrolled Keywords: | enterococcus; human fecal microbiome,ex vivo culture,fos,gos,probiotics,microbiology,molecular medicine,molecular biology,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2400/2404 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 23 Jul 2025 13:30 |
| Last Modified: | 28 Jul 2025 00:32 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/99980 |
| DOI: | 10.1007/s12602-023-10136-9 |
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