Carbon monoxide-oxidising Pseudomonadota on volcanic deposits

Dawson, Robin A., Fantom, Nicola, Martin-Pozas, Tamara, Aguila, Patricia, King, Gary M. and Hernández, Marcela (2025) Carbon monoxide-oxidising Pseudomonadota on volcanic deposits. Environmental Microbiome, 20. ISSN 2524-6372

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Abstract

Carbon monoxide (CO) oxidising microorganisms are present in volcanic deposits throughout succession, with levels of vegetation and soil influencing the communities present. Carboxydovores are a subset of CO oxidisers that use CO as an energy source, which raises questions about the physiological and metabolic features that make them more competitive in harsh volcanic ecosystems. To address these questions, samples were taken from volcanic strata formed by eruptions from Calbuco Volcano (Chile) in 2015 (tephra) and 1917 (soil). Two carboxydovore members of the Burkholderiaceae family were isolated for further study to elucidate the benefits of carboxydovory for the survival of these strains in extreme volcanic ecosystems. The isolates were identified as Paraburkholderia terrae COX (isolated from the 2015 tephra) and Cupriavidus str. CV2 (isolated from the 1917 soil). 16S rRNA gene sequencing showed that within the family Burkholderiacea, the genus Paraburkholderia dominated the 2015 volcanic deposit with an average relative abundance of 73.81%, whereas in the 1917 volcanic deposit, Cupriavidus accounted for 33.64% (average relative abundance). Both strains oxidise CO across a broad range of concentrations (< 100 ppmv – 10,000 ppmv), and genome sequence analysis revealed a candidate form-I carbon monoxide dehydrogenase (CODH), which is likely to catalyse this process. Each strain oxidised CO specifically at stationary phase but the conditions for induction of CODH expression were distinct. Cupriavidus strain CV2 expressed CODH only when CO was added to cultures (100 ppm), while Pb. terrae COX expressed CODH regardless of supplementary CO addition. Based on comparative metabolic and phylogenetic analyses, Cupriavidus strain CV2 is proposed as a novel species within the genus Cupriavidus with the name Cupriavidus ulmosensis sp. nov. for the type strain CV2T (= NCIMB 15506 T, = CECT 30956 T). This study provides valuable insights into the physiology and metabolism of carboxydovores which colonise volcanic ecosystems.

Item Type:	Article
Additional Information:	Availability of data and materials: The genome sequences of the isolates have been deposited in the NCBI GenBank under the accession number PRJNA1001293 (SAMN36798297 for C. ulmosensis CV2T and SAMN36798260 for Pb. terrae COX). The 16S rRNA gene Sanger sequences have been deposited in NCBI under the accession numbers OR536588 for C. ulmosensis CV2T and OR536592 for Pb. terrae COX. The 16S rRNA gene and coxL gene sequencing data of the soils were deposited in the NCBI Sequence Read Archive (SRA) under the Bioproject accession numbers PRJNA1036796 and PRJNA1165783, respectively. Funding: Robin A. Dawson was supported by Royal Society Research Fellows Enhanced grants awarded to Marcela Hernández (RF\ERE\210050 and RF\ERE\231066). Nicola Fantom is funded by a NERC Ph.D. studentship (ARIES-DTP NE/S007334/1). Marcela Hernández was supported by a Royal Society Dorothy Hodgkin Research Fellowship (DHF\R1\211076).
Uncontrolled Keywords:	carbon monoxide,carbon monoxide dehydrogenase,cupriavidus,paraburkholderia,volcanic deposits,applied microbiology and biotechnology,genetics,microbiology ,/dk/atira/pure/subjectarea/asjc/2400/2402
Faculty \ School:	Faculty of Science Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Groups > Wolfson Centre for Advanced Environmental Microbiology
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	23 Jul 2025 11:30
Last Modified:	24 Jul 2025 08:33
URI:	https://ueaeprints.uea.ac.uk/id/eprint/99977
DOI:	10.1186/s40793-025-00672-y

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