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ToolsJuodeikis, Rokas, Jones, Emily, Deery, Evelyne, Beal, David M., Stentz, Régis, Kräutler, Bernhard, Carding, Simon R. and Warren, Martin J. (2022) Nutrient smuggling- Commensal gut bacteria-derived extracellular vesicles scavenge vitamin B12 and related cobamides for microbe and host acquisition. Journal of Extracellular Biology, 1 (10). ISSN 2768-2811
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Abstract
The processes by which bacteria proactively scavenge essential nutrients in crowded environments such as the gastrointestinal tract are not fully understood. In this context, we observed that bacterial extracellular vesicles (BEVs) produced by the human commensal gut microbe Bacteroides thetaiotaomicron contain multiple high-affinity vitamin B12 binding proteins suggesting that the vesicles play a role in micronutrient scavenging. Vitamin B12 belongs to the cobamide family of cofactors that regulate microbial communities through their limited bioavailability. We show that B. thetaiotaomicron derived BEVs bind a variety of cobamides and not only deliver them back to the parental bacterium but also sequester the micronutrient from competing bacteria. Additionally, Caco-2 cells, representing a model intestinal epithelial barrier, acquire cobamide-bound vesicles and traffic them to lysosomes, thereby mimicking the physiological cobalamin-specific intrinsic factor-mediated uptake process. Our findings identify a novel cobamide binding activity associated with BEVs with far-reaching implications for microbiota and host health.
| Item Type: | Article |
|---|---|
| Additional Information: | Publisher Copyright: © 2022 The Authors. Journal of Extracellular Biology published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles. |
| Uncontrolled Keywords: | bacterial extracellular vesicles,bacteroides,cobamides,microbe–host interaction,nutrient uptake,vitamin b,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1307 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School Faculty of Science |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 22 Jul 2025 13:30 |
| Last Modified: | 22 Jul 2025 13:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/99965 |
| DOI: | 10.1002/jex2.61 |
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