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ToolsDezalay, Jordan, Ashworth, Eleanor K., Fulker, Jack Edward, Stockett, Mark H., Noble, Jennifer Anna and Bull, James N. (2025) Cryogenic action spectroscopy of the cyan fluorescent protein chromophore anion. Physical Chemistry Chemical Physics, 27 (32). pp. 16738-16743. ISSN 1463-9076
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Abstract
Action spectroscopy at T ≈ 30 K, as a proxy for the visible absorption band, and the branching between electron detachment and dissociation in the cyan fluorescent protein chromophore anion are reported. The cryogenic action spectra, which show the presence of several rotamers, serve as a reference point for interpreting the effect of nano-environmental interactions in complex protein environments. The adiabatic detachment energy for the lowest energy geometric isomer (Z1) is 19 531 ± 40 cm −1, with the vertical S 1 ← S 0 transition energy at 23 734 ± 40 cm −1. For Z1, the propensity for internal conversion followed by dissociation is low (<10%) compared with autodetachment as the S 1 ← S 0 absorption band is entirely situated in the detachment continuum and is classified as a shape resonance.
| Item Type: | Article |
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| Additional Information: | Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request. Acknowledgements: This work was funded by an EPSRC New Investigator Award (EP/W018691/1 to JNB) and the Swedish Foundation for International Cooperation in Research and Higher Education (STINT) Grant for Internationalisation programme (PT2017-7328 to MHS and JNB). EKA thanks the University of East Anglia for a doctoral studentship. |
| Faculty \ School: | Faculty of Science Faculty of Science > School of Chemistry, Pharmacy and Pharmacology |
| UEA Research Groups: | Faculty of Science > Research Groups > Centre for Photonics and Quantum Science Faculty of Science > Research Groups > Chemistry of Light and Energy |
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| Depositing User: | LivePure Connector |
| Date Deposited: | 17 Jul 2025 10:35 |
| Last Modified: | 28 Aug 2025 03:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/99950 |
| DOI: | 10.1039/D5CP02520F |
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