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ToolsRoy, Shamik, King, Gary M. and Hernández, Marcela (2025) Classification of MAGs associated with trace gas metabolism in volcanic soils named following SeqCode rules. Systematic and Applied Microbiology, 48 (4). ISSN 0723-2020
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Abstract
Trace gas metabolism is important for nutrient flow in all ecosystems, particularly volcanic ecosystems. Microbes in volcanic ecosystems are among the early colonisers and can play key roles in ecological succession. Here, we describe the taxonomic and functional characteristics of two new metagenome-assembled genomes (MAGs), one belonging to Bacteria (MAG_1957-2.1) and one to Archaea (MAG_C2-3), retrieved from soils in volcanoes located in Chile (Llaima) and the USA (Kilauea), respectively. MAG_1957-2.1 has a genome size of 6.36 Mb with 96.21 % completeness. MAG_C2-3 has a genome size of 3.02 Mb with 97.57 % completeness. Phylogenetic analyses of the bacterial MAGs placed MAG_1957-2.1 in the class Ktedonobacteria, while the archaeal MAG_C2-3 was placed in the class Nitrososphaeria. Functional characterisation for potential trace gas metabolism showed that MAG_1957-2.1 contains a coxL gene encoding the large subunit of form I carbon monoxide dehydrogenase (CoxL), which is associated with the oxidation of carbon monoxide (CO). It also contains the form I cox gene cluster with a coxMSL arrangement. On the other hand, MAG_C2-3 contains gene subunit A (amoA) as well as subunit B (amoB), which encode for ammonia monooxygenase, the enzyme that catalyses the oxidation of ammonia. Based on the sequence characteristics and phylogenomic analyses we propose the names Paraktedonobacter carboxidivorans sp. nov for MAG_1957-2.1 and Nitrososphaera maunauluensis sp. nov for MAG_C2-3. The names are proposed following the rules of the published Code of Nomenclature of Prokaryotes Described from Sequence Data (SeqCode).
| Item Type: | Article |
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| Additional Information: | Data availability: Bacterial MAG_1957-2.1 has been deposited in the NCBI-SRA under the bioproject accession number PRJNA602601 (Hernández et al., 2020b). Archaeal MAG-C2-3 has been deposited in the NCBI-SRA under the bioproject accession number PRJNA1096838. The 16S rRNA gene sequences of these MAGs were deposited in the NCBI-SRA under SRA accession numbers SRR32116977 for MAG_1957-2.1 and SRR32116972 for MAG MAG-C2-3. Funding: Shamik Roy was supported by a NERC Discipline Hopping (DH) for Discovery Science grant awarded to Marcela Hernández (NE/X018180/1) and a postdoctoral fellowship from European Union (ERC, BIOCOMP, GA 101075426). Marcela Hernández was supported by a Royal Society Dorothy Hodgkin Research Fellowship (DHF\R1\211076) and Royal Society Research Fellows Enhanced grants (RF\ERE\210050 and RF\ERE\231066). |
| Uncontrolled Keywords: | archaea,chloroflexota,co-dehydrogenase,seqcode,trace gases,volcanic soils,microbiology,ecology, evolution, behavior and systematics,applied microbiology and biotechnology ,/dk/atira/pure/subjectarea/asjc/2400/2404 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Wolfson Centre for Advanced Environmental Microbiology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 30 Jun 2025 10:30 |
| Last Modified: | 01 Jul 2025 03:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/99766 |
| DOI: | 10.1016/j.syapm.2025.126622 |
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