Evaluating different extraction solvents for GC-MS based metabolomic analysis of the fecal metabolome of adult and baby giant pandas

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Yang, Yang, Yin, Yanqiang, Chen, Xuyang, Chen, Chang, Xia, Yinyin, Qi, Hongbo, Baker, Philip N., Zhang, Hua and Han, Ting Li (2019) Evaluating different extraction solvents for GC-MS based metabolomic analysis of the fecal metabolome of adult and baby giant pandas. Scientific Reports, 9 (1). ISSN 2045-2322

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Abstract

The gut microbiome plays a fundamental role in host health and the fecal metabolome can be analysed to assess microbial activity and can be used as an intermediate phenotype monitoring the host-microbiome relationship. However, there is no established extraction protocol to study the fecal metabolome of giant pandas. The aim of this research is to optimize extraction of the fecal metabolome from adult and baby pandas for high throughput metabolomics analysis using gas chromatography-mass spectrometry (GC-MS). Fecal samples were collected from eight adult pandas and a pair of twin baby pandas. Six different extraction solvents were investigated and evaluated for their reproducibility, metabolite coverage, and extraction efficiency, particularly in relation to the biochemical compound classes such as amino acids, tricarboxylic acid (TCA) cycle intermediates, fatty acids, secondary metabolites, and vitamin and cofactors. Our GC-MS results demonstrated that the extraction solvents with isopropanol: acetonitrile: water (3:2:2 ratio) and 80% methanol were the most appropriate for studying the fecal metabolome of adult and baby giant pandas respectively. These extraction solvents can be used in future study protocols for the analysis of the fecal metabolome in giant pandas.

Item Type: Article
Additional Information: Data Availability: The data that support the findings of this study are available from the corresponding author upon reasonable request. Funding information: This research was supported by the National Natural Science Foundation of China (No. 81871185, 81571453, 81771607, 81701477), The 111 Project (Yuwaizhuan (2016)32 The National Key Research and Development Program of Reproductive Health & Major Birth Defects Control and Prevention (2016YFC1000407), Health Commission (2018ZDXM024, 2017ZDXM008), and Chongqing Science & Technology Commission (cstc2017jcyjBX0062).
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 23 Jun 2025 09:31
Last Modified: 24 Jun 2025 13:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/99662
DOI: 10.1038/s41598-019-48453-1

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