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ToolsAitken-Rogers, Helen, Singleton, Chloe, Lewin, Allison, Taylor-Gee, Alice, Moore, Geoffrey R. and Le Brun, Nick E. (2004) Effect of phosphate on bacterioferritin-catalysed iron(II) oxidation. Journal of Biological Inorganic Chemistry, 9 (2). pp. 161-170. ISSN 0949-8257
Full text not available from this repository.Abstract
The iron(III) mineral cores of bacterioferritins (BFRs), as isolated, contain a significant component of phosphate, with an iron-to-phosphate ratio approaching 1:1 in some cases. In order to better understand the in vivo core-formation process, the effect of phosphate on in vitro core formation in Escherichia coli BFR was investigated. Iron cores reconstituted in the presence of phosphate were found to have iron-to-phosphate ratios similar to those of native cores, and possessed electron paramagnetic resonance properties characteristic of the phosphate-rich core. Phosphate did not affect the stoichiometry of the initial iron(II) oxidation reaction that takes place at the intrasubunit dinuclear iron-binding sites (phase 2 of core formation), but did increase the rate of oxidation. Phosphate had a more significant effect on subsequent core formation (the phase 3 reaction), increasing the rate up to five-fold at pH 6.5 and 25 °C. The dependence of the phase 3 rate on phosphate was complex, being greatest at low phosphate and gradually decreasing until the point of saturation at ∼2 mM phosphate (for iron(II) concentrations < 200 μM). Phosphate caused a significant decrease in the absorption properties of both phase 2 and phase 3 products, and the phosphate dependence of the latter mirrored the observed rate dependence, suggesting that distinct iron(III)-phosphate species are formed at different phosphate concentrations. The effect of phosphate on absorption properties enabled the observation of previously undetected events in the phase 2 to phase 3 transition period.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | bacterioferritins,ferritins,ferroxidase,iron core,phosphate,biochemistry,inorganic chemistry ,/dk/atira/pure/subjectarea/asjc/1300/1303 |
| Faculty \ School: | Faculty of Science > School of Chemistry (former - to 2024) Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Chemistry of Life Processes Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 18 Jun 2025 08:30 |
| Last Modified: | 18 Jun 2025 08:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/99594 |
| DOI: | 10.1007/s00775-003-0504-1 |
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