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ToolsChen, Jingdong, Han, Ting-Li, Zhou, Xiaobo, Baker, Philip, Shao, Yong and Zhang, Hua (2020) Metabolic disparities of different oxidative stress-inducing conditions in HTR8/SVneo cells. Molecular Medicine Reports, 21 (2). pp. 540-548. ISSN 1791-2997
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Abstract
Placental oxidative stress is present throughout the duration of pregnancy, but it is when oxidative stress exceeds the normal physiological level that complications can occur. Trophoblast cell lines are commonly utilized for oxidative stress research due to their distinct uniform cell population and easy-to-apply interventions. However, conflicting results are often reported when different oxidative stress cell models are used. in this study, the aim was to characterize the intracellular and extracellular metabolite profiles of different oxidative stress cell models commonly used in the research of pregnancy complications. HTr8/SVneo human trophoblast cell lines were treated with five different oxidative stress-inducing conditions: Hypoxia (1% oxygen); hypoxia and reoxygenation; cobalt chloride (cocl2; 300 µmol/l); sodium nitroprusside (SnP; 2.5 mmol/l); and the serum of women with preeclampsia (10% v/v). intracellular metabolites were extracted from cells and extracellular metabolites were collected from spent media for metabolomic analysis via gas chromatography-mass spectrometry. The results demonstrated that there were distinct differences in the intracellular and extracellular metabolome between the different cell models. Meanwhile, treatments with exogenous drugs, such as cocl2 and SNP, resulted in more similar metabolite profiles. These disparities between the different oxidative stress cell models will have implications for the applications of these results, and highlight the need for the standardization of oxidative stress cell models in obstetric research.
| Item Type: | Article |
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| Additional Information: | Availability of data and materials: The datasets used and analyzed during the current study are available from the corresponding author on reasonable request. Funding information: The present study was supported by The National Natural Science Foundation of China (grant nos. 81571453, 81771607, 81871185, 81701477 and 81471473). The 111 Project [grant no. Yuwaizhuan (2016)32], The National Key Research and Development Program of Reproductive Health & Major Birth Defects Control and Prevention (grant no. 2016YFC1000407), Chongqing Health Commission (grant nos. 2017ZDXM008 and 2018ZDXM024), and Chongqing Science & Technology Commission (grant no. cstc2017jcyjBX0062). |
| Uncontrolled Keywords: | cobalt chloride,hypoxia,metabolomics,oxidative stress,preeclampsia,sodium nitroprusside,biochemistry,molecular medicine,molecular biology,genetics,oncology,cancer research,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/1300/1303 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 11 Jun 2025 15:30 |
| Last Modified: | 23 Jun 2025 09:31 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/99480 |
| DOI: | 10.3892/mmr.2019.10861 |
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