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ToolsTian, Jing, Liu, Yamin, Hu, Mingyu, Zheng, Yangxi, Xu, Ping, Zhang, Lan, Liao, Jiujiang, Wu, Yue, Wen, Li, Tong, Chao, Yan, Jianying, Qi, Hongbo, Saffery, Richard, Baker, Philip N. and Kilby, Mark D. (2020) Upregulated LncZBTB39 in pre-eclampsia and its effects on trophoblast invasion and migration via antagonizing the inhibition of miR-210 on THSD7A expression. European Journal of Obstetrics and Gynecology and Reproductive Biology, 248. pp. 164-171. ISSN 0301-2115
Full text not available from this repository. (Request a copy)Abstract
Objective: Pre-eclampsia (PE) is a major cause of maternal morbidity and mortality, but its etiology remains to be elucidated. Accumulating evidence suggests that placental long noncoding RNAs (lncRNAs) might contribute to the pathogenesis of pre-eclampsia. Study Design: In the present study, the expression levels of lncRNAs in human placenta were first determined by microarray analysis and then validated by secondary RT-qPCR and FISH. LncZBTB39 expression manipulation in HTR8/SVneo trophoblast cells was achieved by shRNA and plasmid transfection. Then, the invasion and migration of lncZBTB39-deficient and lncZBTB39-overexpressing trophoblast cells were evaluated by transwell assays and wound-healing assays, respectively. MMP2 activity was measured by gelatin zymography. The downstream target genes of lncZBTB39 were then identified by a transcriptomic microarray, followed by RT-qPCR validation. Results: We found that lncZBTB39 was upregulated in PE-complicated human placentas, and overexpression of lncZBTB39 inhibited invasion and migration, as well as MMP2 activity in HTR8/SVneo cells, while downregulation of lncZBTB39 enhanced invasion, migration and MMP2 activity. In addition, THSD7A expression was elevated by lncZBTB39 overexpression but reduced in lncZBTB39-deficient cells; moreover, lncZBTB39 antagonized the inhibitory effects of miR-210 on THSD7A expression. Conclusion: PE-complicated placentas are associated with upregulated lncZBTB39, which negatively regulates trophoblast invasion and migration, most likely by preserving the expression of THSD7A mRNA through sponging miR-210. The results of this study not only provide novel evidence that lncRNAs regulate trophoblastic activities but also suggest that lncZBTB39 may be a potential interventional target for PE.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | invasion,lnczbtb39,pre-eclampsia,trophoblast,reproductive medicine,obstetrics and gynaecology,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2700/2743 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 11 Jun 2025 14:30 |
| Last Modified: | 13 Jun 2025 15:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/99473 |
| DOI: | 10.1016/j.ejogrb.2020.03.035 |
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