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ToolsAllsopp, Rebecca C., Cowley, Caroline M., Barber, Ruth C., Jones, Carolyn, Holmes, Christopher W., Bird, Paul W., Gohil, Shailesh G., Blackmore, Claire, Tobin, Martin D., Brunskill, Nigel, Baker, Philip N. and Shaw, Jacqui A. (2022) A rapid RT-LAMP SARS-CoV-2 screening assay for collapsing asymptomatic COVID-19 transmission. PLoS One, 17 (9). ISSN 1932-6203
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Abstract
Purpose To demonstrate the diagnostic performance of rapid SARS-CoV-2 RT-LAMP assays, comparing the performance of genomic versus sub-genomic sequence target with subsequent application in an asymptomatic screening population. Methods RT-LAMP diagnostic specificity (DSe) and sensitivity (DSe) was determined using 114 RT-PCR clinically positive and 88 RT-PCR clinically negative swab samples processed through the diagnostic RT-PCR service within the University Hospitals of Leicester NHS Trust. A swab-based RT-LAMP SARS-CoV-2 screening programme was subsequently made available to all staff and students at the University of Leicester (Autumn 2020), implemented to ISO 15189:2012 standards using NHS IT infrastructure and supported by University Hospital Leicester via confirmatory NHS diagnostic laboratory testing of RT-LAMP ‘positive’ samples. Results Validation samples reporting a Ct < 20 were detected at 100% DSe and DSp, reducing to 95% DSe (100% DSp) for all samples reporting a Ct < 30 (both genomic dual sub-genomic assays). Advisory screening identified nine positive cases in 1680 symptom free individuals (equivalent to 540 cases per 100,000) with results reported back to participants and feed into national statistics within 48 hours. Conclusion This work demonstrates the utility of a rapid RT-LAMP assay for collapsing transmission of SARS-CoV-2 in an asymptomatic screening population.
| Item Type: | Article |
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| Additional Information: | Data Availability: All relevant data are within the paper and its Supporting information files. Funding: Assay validation supported by Leicester Drug Discovery and Diagnostics using MRC Confidence in Concept Award funds (MRC Award number MC- MC_PC_18054). The Screening Programme for Autumn Term 2020 supported by core funding from the University of Leicester. This research was in part funded by the Wellcome Trust (award number WT202849/Z/16/Z) and NIHR Leicester Biomedical Research Centre (award number NIHR201371); the views expressed are those of the author(s) and not necessarily those of the NHS, the NIHR or the Department of Health. For the purpose of open access, the author has applied a CC BY public copyright license to any Author Accepted Manuscript version arising from this submission. M.D.T. holds an NIHR Senior Investigator Award (NIHR201371) and a Wellcome Trust Investigator Award (WT202849/Z/16/Z). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
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| Depositing User: | LivePure Connector |
| Date Deposited: | 08 May 2025 08:34 |
| Last Modified: | 08 May 2025 08:34 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/99210 |
| DOI: | 10.1371/journal.pone.0273912 |
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