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ToolsFlotte de Pouzols, James, Subramani, Arun, Ashworth, Eleanor K., Bull, James N., Cederquist, Henrik, Dezalay, Jordan, List, Nanna Holmgaard, Martini, Paul, Navarrete, José E. Navarro, Schmidt, Henning T., Zettergren, Henning, Zhu, Boxing and Stockett, Mark H. (2025) Radiative cooling of the deprotonated cyan fluorescent protein chromophore anion. Physical Review A, 111 (4). ISSN 1050-2947
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Abstract
An electrospray ion source has been coupled to a cryogenic electrostatic ion-beam storage ring to enable experimental studies of the fundamental properties of biomolecular ions and their reactions in the gas phase on longer timescales than with previous instruments. Using this equipment, we have measured the vibrational radiative cooling rate of the deprotonated anion of the chromophore of the cyan fluorescent protein, a color-shifted mutant of the iconic green fluorescent protein. Time-resolved dissociation rates of collisionally activated ions are first measured to benchmark a model of the dissociation rate coefficient. Storage time-dependent laser-induced dissociation rates are then measured to probe the evolution of the internal energy distribution of the stored ion ensemble. We find that significant heating of the electrosprayed ions occurs upon their extraction from the ion source, and that the radiative cooling rate is consistent with the prediction of a simple harmonic cascade model of vibrational relaxation.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding information: This work was supported by Swedish Research Council Grants No. 2016-03675 (M.H.S.), No. 2018-04092 (H.T.S.), No. 2023-03833 (H.C.), No. 2020-03437 (H.Z.), Knut and Alice Wallenberg Foundation Grant No. 2018.0028 (H.C., H.T.S., and H.Z.), EPSRC Grant No. EP/W018691 (J.N.B.), Olle Engkvist Foundation Grant No. 200-575 (M.H.S.), and Swedish Foundation for International Collaboration in Research and Higher Education (STINT) Grant No. PT2017-7328 (J.N.B. and M.H.S.). We acknowledge the DESIREE infrastructure for the provision of facilities and experimental support, and we thank the operators and technical staff for their invaluable assistance. The DESIREE infrastructure receives funding from the Swedish Research Council under Grants No. 2021-00155 and No. 2023-00170. This article is based upon work from COST Action CA21126–Carbon molecular nanostructures in space (NanoSpace), supported by COST (European Cooperation in Science and Technology). |
| Faculty \ School: | Faculty of Science Faculty of Science > School of Chemistry, Pharmacy and Pharmacology |
| UEA Research Groups: | Faculty of Science > Research Groups > Centre for Photonics and Quantum Science Faculty of Science > Research Groups > Chemistry of Light and Energy |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 23 Apr 2025 16:30 |
| Last Modified: | 27 Apr 2025 06:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/99064 |
| DOI: | 10.1103/PhysRevA.111.043112 |
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