Utilising human induced pluripotent stem cells (hiPSCs) to investigate endocardial cell biology

Collins, Amelia (2024) Utilising human induced pluripotent stem cells (hiPSCs) to investigate endocardial cell biology. Masters thesis, University of East Anglia.

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Abstract

Culture and differentiation methods of induced pluripotent stem cells (iPSCs) have shifted from uncontrolled systems to those which are more streamlined and robust. Chemically defined systems have allowed for the standardisation and reduced variability of protocols, thus allowing for a more efficient derivation of desired cell types. While the field of cardiovascular stem cell science is primarily focused on iPSC-derived cardiomyocytes, deriving other cardiac cell types such as endocardial cells may prove to be pivotal in the holistic understanding of cardiovascular pathology. In this project, we aim to generate a protocol to derive endocardial cells from human iPSCs. We also aim to generate and utilise genetic constructs to aid our optimisation of the protocol.

Item Type:	Thesis (Masters)
Faculty \ School:	Faculty of Science > School of Biological Sciences
Depositing User:	Chris White
Date Deposited:	25 Mar 2025 08:25
Last Modified:	25 Mar 2025 08:25
URI:	https://ueaeprints.uea.ac.uk/id/eprint/98881
DOI:

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