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ToolsKlein, Timothy, Hodgskiss, Logan H., Dreer, Max, Murrell, J. Colin, Hutchings, Matthew I., Schleper, Christa and Lehtovirta-Morley, Laura E. (2025) Distinct patterns of antibiotic sensitivities in ammonia-oxidising archaea. Environmental Microbiology, 27 (3). ISSN 1462-2912
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Abstract
Ammonia-oxidising archaea (AOA) are important microorganisms contributing towards the nitrogen flux in the environment. Unlike archaea from other major phyla, genetic tools are yet to be developed for the AOA, and identification of antibiotic resistance markers for selecting mutants is required for a genetic system. The aim of this study was to test the effects of selected antibiotics (hygromycin B, neomycin, apramycin, puromycin, novobiocin) on pure cultures of three well studied AOA strains, ‘Candidatus Nitrosocosmicus franklandianus C13’, Nitrososphaera viennensis EN76 and Nitrosopumilus maritimus SCM1. Puromycin, hygromycin B and neomycin inhibited some but not all tested archaeal strains. All strains were resistant to apramycin and inhibited by novobiocin to various degrees. As N. viennensis EN76 was relatively more resistant to the tested antibiotics, a wider range of concentrations and compounds (chloramphenicol, trimethoprim, statins) was tested against this strain. N. viennensis EN76 was inhibited by trimethoprim, but not by chloramphenicol, and growth recovered within days in the presence of simvastatin, suggesting either degradation of, or spontaneous resistance against, this compound. This study highlights the physiological differences between different genera of AOA and has identified new candidate antibiotics for selective enrichment and the development of selectable markers for genetic systems in AOA.
| Item Type: | Article |
|---|---|
| Additional Information: | Data Availability Statement: Data are available from the corresponding author upon reasonable request. Funding information: HORIZON EUROPE European Research Council. Grant Number: ActionR 101079299; European Research Council. Grant Number: UNITY 852993; Austrian Science Fund. Grant Number: W1257; Natural Environment Research Council. Grant Number: NE/S007334/1; Royal Society. Grant Number: DH150187 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Science > School of Environmental Sciences University of East Anglia Research Groups/Centres > Theme - ClimateUEA |
| UEA Research Groups: | Faculty of Science > Research Groups > Environmental Biology Faculty of Science > Research Groups > Molecular Microbiology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 20 Mar 2025 17:30 |
| Last Modified: | 28 Mar 2025 13:15 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/98861 |
| DOI: | 10.1111/1462-2920.70063 |
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