First evidence of production of the lantibiotic nisin P

Garcia-Gutierrez, Enriqueta, O’Connor, Paula M., Saalbach, Gerhard, Walsh, Calum J., Hegarty, James W., Guinane, Caitriona M., Mayer, Melinda J., Narbad, Arjan and Cotter, Paul D. (2020) First evidence of production of the lantibiotic nisin P. Scientific Reports, 10. ISSN 2045-2322

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Abstract

Nisin P is a natural nisin variant, the genetic determinants for which were previously identified in the genomes of two Streptococcus species, albeit with no confirmed evidence of production. Here we describe Streptococcus agalactiae DPC7040, a human faecal isolate, which exhibits antimicrobial activity against a panel of gut and food isolates by virtue of producing nisin P. Nisin P was purified, and its predicted structure was confirmed by nanoLC-MS/MS, with both the fully modified peptide and a variant without rings B and E being identified. Additionally, we compared its spectrum of inhibition and minimum inhibitory concentration (MIC) with that of nisin A and its antimicrobial effect in a faecal fermentation in comparison with nisin A and H. We found that its antimicrobial activity was less potent than nisin A and H, and we propose a link between this reduced activity and the peptide structure.

Item Type:	Article
Additional Information:	Data availability statement: The authors declare that the data supporting the findings of this study are available within the paper and in Supplementary Table S1. Funding information: This work was supported by Walsh Fellowship under Grant 2015066; BBSRC Institute Strategic Programme under Grant BB/R012490/1 and BBS/E/F/000PR10356; and SFI under Grants SFI/11/PI/1137 and SFI/12/RC/2273.
Faculty \ School:	Faculty of Science > School of Biological Sciences
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	03 Mar 2025 15:30
Last Modified:	28 Mar 2025 13:14
URI:	https://ueaeprints.uea.ac.uk/id/eprint/98637
DOI:	10.1038/s41598-020-60623-0

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