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ToolsSaha, Shikha, Day-Walsh, Priscilla, Shehata, Emad and Kroon, Paul Anthony (2021) Development and validation of a LC-MS/MS technique for the analysis of short chain fatty acids in tissues and biological fluids without derivatisation using isotope labelled internal standards. Molecules, 26 (21). ISSN 1420-3049
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Abstract
The gut microbiota is critical to the maintenance of physiological homeostasis and as such is implicated in a range of diseases such as colon cancer, ulcerative colitis, diabetes, cardiovascular diseases, and neurodegenerative diseases. Short chain fatty acids (SCFAs) are key metabolites produced by the gut microbiota from the fermentation of dietary fibre. Here we present a novel, sensitive, and direct LC-MS/MS technique using isotopically labelled internal standards without derivatisation for the analysis of SCFAs in different biological matrices. The technique has significant advantages over the current widely used techniques based on sample derivatization and GC-MS analysis, including fast and simple sample preparation and short LC runtime (10 min). The technique is specific and sensitive for the quantification of acetate, butyrate, isobutyrate, isovalerate, lactate, propionate and valerate. The limits of detection were all 0.001 mM except for acetate which was 0.003 mM. The calibration curves for all the analytes were linear with correlation coefficients r2 > 0.998. The intra-and inter-day precisions in three levels of known concentrations were <12% and <20%, respectively. The quantification accuracy ranged from 92% to 120%. The technique reported here offers a valuable analytical tool for use in studies of SCFA production in the gut and their distribution to host tissues.
| Item Type: | Article |
|---|---|
| Additional Information: | Data Availability Statement: The data presented in this study are available on request from the corresponding author. |
| Uncontrolled Keywords: | acetate,butyrate,cardiovascular,diabetes,gut microbiota,kidney,lactate,milk,neurodegenerative,plasma,propionate,analytical chemistry,chemistry (miscellaneous),molecular medicine,pharmaceutical science,drug discovery,physical and theoretical chemistry,organic chemistry ,/dk/atira/pure/subjectarea/asjc/1600/1602 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 18 Feb 2025 14:30 |
| Last Modified: | 20 Feb 2025 01:20 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/98538 |
| DOI: | 10.3390/molecules26216444 |
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