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ToolsHawkins, Erica, Chen, Jiawen, Watson-Lazowski, Alexander, Ahn-Jarvis, Jennifer, Barclay, J. Elaine, Fahy, Brendan, Hartley, Matthew, Warren, Frederick J. and Seung, David (2021) STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endosperm. New Phytologist, 230 (6). pp. 2371-2386. ISSN 0028-646X
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Abstract
Starch granule initiation is poorly understood at the molecular level. The glucosyltransferase, STARCH SYNTHASE 4 (SS4), plays a central role in granule initiation in Arabidopsis leaves, but its function in cereal endosperms is unknown. We investigated the role of SS4 in wheat, which has a distinct spatiotemporal pattern of granule initiation during grain development. We generated TILLING mutants in tetraploid wheat (Triticum turgidum) that are defective in both SS4 homoeologs. The morphology of endosperm starch was examined in developing and mature grains. SS4 deficiency led to severe alterations in endosperm starch granule morphology. During early grain development, while the wild-type initiated single ‘A-type’ granules per amyloplast, most amyloplasts in the mutant formed compound granules due to multiple initiations. This phenotype was similar to mutants deficient in B-GRANULE CONTENT 1 (BGC1). SS4 deficiency also reduced starch content in leaves and pollen grains. We propose that SS4 and BGC1 are required for the proper control of granule initiation during early grain development that leads to a single A-type granule per amyloplast. The absence of either protein results in a variable number of initiations per amyloplast and compound granule formation.
| Item Type: | Article |
|---|---|
| Additional Information: | Data availability statement: The data that support the findings of this study are available from the corresponding author upon reasonable request. |
| Uncontrolled Keywords: | amyloplast,bgc1,endosperm,granule initiation,ss4,starch,starch synthesis,wheat,physiology,plant science ,/dk/atira/pure/subjectarea/asjc/1300/1314 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Medicine and Health Sciences > Norwich Medical School Faculty of Science > School of Computing Sciences Faculty of Science > School of Chemistry, Pharmacy and Pharmacology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 18 Feb 2025 10:30 |
| Last Modified: | 18 Feb 2025 10:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/98531 |
| DOI: | 10.1111/nph.17342 |
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