Characterisation of the introgression of Brassica villosa genome into broccoli to enhance methionine-derived glucosinolates and associated health benefits

Neequaye, Mikhaela, Steuernagel, Burkhard, Saha, Shikha, Trick, Martin, Troncoso-Rey, Perla, van den Bosch, Frans, Traka, Maria H., Østergaard, Lars and Mithen, Richard (2022) Characterisation of the introgression of Brassica villosa genome into broccoli to enhance methionine-derived glucosinolates and associated health benefits. Frontiers in Plant Science, 13. ISSN 1664-462X

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Abstract

Broccoli cultivars that have enhanced accumulation of methionine-derived glucosinolates have been developed through the introgression of a novel allele of the MYB28 transcription factor from the wild species Brassica villosa. Through a novel k-mer approach, we characterised the extent of the introgression of unique B. villosa genome sequences into high glucosinolate broccoli genotypes. RNAseq analyses indicated that the introgression of the B. villosa MYB28 C2 allele resulted in the enhanced expression of the MYB28 transcription factor, and modified expression of genes associated with sulphate absorption and reduction, and methionine and glucosinolate biosynthesis when compared to standard broccoli. A adenine-thymine (AT) short tandem repeat (STR) was identified within the 5′ untranslated region (UTR) B. villosa MYB28 allele that was absent from two divergent cultivated forms of Brassica oleracea, which may underpin the enhanced expression of B. villosa MYB28.

Item Type:	Article
Additional Information:	Data Availability Statement: The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: National Center for Biotechnology Information (NCBI) BioProject database under accession number PRJNA623495. Funding Information: This work was supported by the UKRI Biotechnology and Biological Sciences Research Council (BBSRC) through institute strategic programmes BB/P013511/1 to the John Innes Centre and BB/R012512/1 and its constituent project(s) BBS/E/F/000PR10343 to the Quadram Institute. MN was supported by the BBSRC Norwich Research Park Biosciences Doctoral Training Partnership grant number BB/M011216/1.
Uncontrolled Keywords:	brassica oleracea,broccoli,glucoraphanin,introgression,myb28,sulphur metabolism,transcription factor,transcriptome,plant science ,/dk/atira/pure/subjectarea/asjc/1100/1110
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Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	18 Feb 2025 10:30
Last Modified:	19 Feb 2025 13:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/98529
DOI:	10.3389/fpls.2022.855707

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