Structural and mechanistic basis of immunity toward endonuclease colicins

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Kleanthous, Colin, Kühlmann, Ulrike C., Pommer, Ansgar J., Ferguson, Neil, Radford, Sheena E., Moore, Geoffrey R., James, Richard and Hemmings, Andrew M. (1999) Structural and mechanistic basis of immunity toward endonuclease colicins. Nature Structural Biology, 6. pp. 243-252. ISSN 1072-8368

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Abstract

The crystal structure of the cytotoxic endonuclease domain from the bacterial toxin colicin E9 in complex with its cognate immunity protein Im9 reveals that the inhibitor does not bind at the active site, the core of which comprises the HNH motif found in intron-encoded homing endonucleases, but rather at an adjacent position leaving the active site exposed yet unable to bind DNA because of steric and electrostatic clashes with incoming substrate. Although its mode of action is unorthodox, Im9 is a remarkably effective inhibitor since it folds within milliseconds and then associates with its target endonuclease at the rate of diffusion to form an inactive complex with sub-femtomolar binding affinity. This hyperefficient mechanism of inhibition could be well suited to other toxic enzyme systems, particularly where the substrate is a polymer extending beyond the boundaries of the active site.

Item Type:	Article
Uncontrolled Keywords:	structural biology,biochemistry,genetics ,/dk/atira/pure/subjectarea/asjc/1300/1315
Faculty \ School:	Faculty of Science > School of Chemistry, Pharmacy and Pharmacology Faculty of Science > School of Biological Sciences
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	13 Feb 2025 17:30
Last Modified:	28 Mar 2025 13:12
URI:	https://ueaeprints.uea.ac.uk/id/eprint/98476
DOI:	10.1038/6683

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