Crystallization of and preliminary X-ray data for the negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa

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Wilson, Stuart A., Chayen, Naomi E., Hemmings, Andrew M., Drew, Robert E. and Pearl, Laurence H. (1991) Crystallization of and preliminary X-ray data for the negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa. Journal of Molecular Biology, 222 (4). pp. 869-871. ISSN 0022-2836

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Abstract

The negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa has been purified from an over-expressing clone and crystalized. Crystals of diffraction quality were obtained from polyethylene glycol 4000 and ammonium sulphate. AmiC crystallizes in P42212 (a = 104·4 A ̊, c = 66·6 A ̊) with one subunit in the asymmetric unit. Crystals diffract beyond 2·8 Å.

Item Type:	Article
Uncontrolled Keywords:	amidase,anti-termination mechanism,catabolic operon,negative regulator,p. aeruginosa,biophysics,structural biology,molecular biology ,/dk/atira/pure/subjectarea/asjc/1300/1304
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	13 Feb 2025 15:30
Last Modified:	16 Feb 2025 06:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/98470
DOI:	10.1016/0022-2836(91)90579-U

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