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ToolsLi, Chun-Yang, Cao, Hai-Yan, Wang, Qing, Carrión, Ornella, Zhu, Xiaoyu, Miao, Jie, Wang, Peng, Chen, Xiu-Lan, Todd, Jonathan D. and Zhang, Yu-Zhong (2023) Aerobic methylation of hydrogen sulfide to dimethylsulfide in diverse microorganisms and environments. ISME Journal, 17 (8). pp. 1184-1193. ISSN 1751-7362
Full text not available from this repository. (Request a copy)Abstract
Dimethylsulfide (DMS) is the major biosulfur source emitted to the atmosphere with key roles in global sulfur cycling and potentially climate regulation. The main precursor of DMS is thought to be dimethylsulfoniopropionate. However, hydrogen sulfide (H2S), a widely distributed and abundant volatile in natural environments, can be methylated to DMS. The microorganisms and the enzymes that convert H2S to DMS, and their importance in global sulfur cycling were unknown. Here we demonstrate that the bacterial MddA enzyme, previously known as a methanethiol S-methyltransferase, could methylate inorganic H2S to DMS. We determine key residues involved in MddA catalysis and propose the mechanism for H2S S-methylation. These results enabled subsequent identification of functional MddA enzymes in abundant haloarchaea and a diverse range of algae, thus expanding the significance of MddA mediated H2S methylation to other domains of life. Furthermore, we provide evidence for H2S S-methylation being a detoxification strategy in microorganisms. The mddA gene was abundant in diverse environments including marine sediments, lake sediments, hydrothermal vents and soils. Thus, the significance of MddA-driven methylation of inorganic H2S to global DMS production and sulfur cycling has likely been considerably underestimated.
| Item Type: | Article |
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| Additional Information: | Funding Information: We thank Terry McGenity from University of Essex for providing the Haladaptatus sp. W1 strain, and Emese Bartha from University of East Anglia for providing advice on how to culture it. This work was supported by the Marine S&T Fund of Shandong Province for Qingdao Marine Science and Technology Center (No. 2022QNLM030004-3), the National Key Research and Development Program of China (2022YFC2807500), the National Science Foundation of China (grants 42276102, 92251303, 42076229, 31961133016), the Fundamental Research Funds for the Central Universities (202172002, 202041011), the Major Scientific and Technological Innovation Project (MSTIP) of Shandong Province (2019JZZY010817), the Program of Shandong for Taishan Scholars (tspd20181203), the Biotechnology and Biological Sciences Research Council, UK, grant (BB/X005968), Natural Environment Research Council, UK, Standard grants (NE/X000990, NE/V000756 and NE/S001352) and the Leverhulme Trust research grant (RPG-2020-413). Funding Information: We thank Terry McGenity from University of Essex for providing the Haladaptatus sp. W1 strain, and Emese Bartha from University of East Anglia for providing advice on how to culture it. This work was supported by the Marine S&T Fund of Shandong Province for Qingdao Marine Science and Technology Center (No. 2022QNLM030004-3), the National Key Research and Development Program of China (2022YFC2807500), the National Science Foundation of China (grants 42276102, 92251303, 42076229, 31961133016), the Fundamental Research Funds for the Central Universities (202172002, 202041011), the Major Scientific and Technological Innovation Project (MSTIP) of Shandong Province (2019JZZY010817), the Program of Shandong for Taishan Scholars (tspd20181203), the Biotechnology and Biological Sciences Research Council, UK, grant (BB/X005968), Natural Environment Research Council, UK, Standard grants (NE/X000990, NE/V000756 and NE/S001352) and the Leverhulme Trust research grant (RPG-2020-413). Publisher Copyright: © 2023, The Author(s), under exclusive licence to International Society for Microbial Ecology. |
| Uncontrolled Keywords: | microbiology,ecology, evolution, behavior and systematics,sdg 13 - climate action,sdg 14 - life below water ,/dk/atira/pure/subjectarea/asjc/2400/2404 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Molecular Microbiology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 24 Jan 2025 01:36 |
| Last Modified: | 28 Jan 2025 23:48 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/98304 |
| DOI: |
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