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ToolsAdriaenssens, Evelien M., Farkas, Kata, McDonald, James E., Jones, David L., Allison, Heather E. and McCarthy, Alan J. (2021) Tracing the fate of wastewater viruses reveals catchment-scale virome diversity and connectivity. Water Research, 203. ISSN 0043-1354
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Abstract
The discharge of wastewater-derived viruses in aquatic environments impacts catchment-scale virome composition. To explore this, we used viromic analysis of RNA and DNA virus-like particles to holistically track virus communities entering and leaving wastewater treatment plants and the connecting river catchment system and estuary. We reconstructed >40 000 partial viral genomes into 10 149 species-level groups, dominated by dsDNA and (+)ssRNA bacteriophages (Caudoviricetes and Leviviricetes) and a small number of genomes that could pose a risk to human health. We found substantial viral diversity and geographically distinct virus communities associated with different wastewater treatment plants. River and estuarine water bodies harboured more diverse viral communities in downstream locations, influenced by tidal movement and proximity to wastewater treatment plants. Shellfish and beach sand were enriched in viral communities when compared with the surrounding water, acting as entrapment matrices for virus particles. Extensive phylogenetic analyses of environmental-derived and reference sequences showed the presence of human-associated sapovirus GII in all sample types, multiple rotavirus A strains in wastewater and a diverse set of picorna-like viruses associated with shellfish. We conclude that wastewater-derived viral genetic material is commonly deposited in the environment and can be traced throughout the freshwater-marine continuum of the river catchment, where it is influenced by local geography, weather events and tidal effects. Our data illustrate the utility of viromic analyses for wastewater- and environment-based ecology and epidemiology, and we present a conceptual model for the circulation of all types of viruses in a freshwater catchment.
| Item Type: | Article |
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| Additional Information: | Availability of data and material: The datasets generated and analysed in this study are available from the Sequence Read Archive (SRA) under BioProject PRJNA509142, accession numbers SRR8299359 to SRR8299398. Funding Information: This work was supported by the Natural Environment Research Council (NERC) and the Food Standards Agency (FSA) under the Environmental Microbiology and Human Health (EMHH) Programme (VIRAQUA; NE/M010996/1). E.M.A is currently funded by the Biotechnology and Biological Sciences Research Council (BBSRC) Institute Strategic Programme Gut Microbes and Health (BB/R012490/1) and its constituent projects BBS/E/F/000PR10353 and BBS/E/F/000PR10356. |
| Uncontrolled Keywords: | aquatic viruses,shellfish viruses,viral diversity,viromics,virus ecology,wastewater contamination,wastewater viruses,environmental engineering,civil and structural engineering,ecological modelling,water science and technology,waste management and disposal,pollution ,/dk/atira/pure/subjectarea/asjc/2300/2305 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 16 Jan 2025 01:10 |
| Last Modified: | 19 Jan 2025 01:00 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/98221 |
| DOI: | 10.1016/j.watres.2021.117568 |
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