A single NLR gene confers resistance to leaf and stripe rust in wheat

Sharma, Davinder, Avni, Raz, Gutierrez-Gonzalez, Juan, Kumar, Rakesh, Sela, Hanan, Prusty, Manas Ranjan, Shatil-Cohen, Arava, Molnár, István, Holušová, Kateřina, Said, Mahmoud, Doležel, Jaroslav, Millet, Eitan, Khazan-Kost, Sofia, Landau, Udi, Bethke, Gerit, Sharon, Or, Ezrati, Smadar, Ronen, Moshe, Maatuk, Oxana, Eilam, Tamar, Manisterski, Jacob, Ben-Yehuda, Pnina, Anikster, Yehoshua, Matny, Oadi, Steffenson, Brian J., Mascher, Martin, Brabham, Helen J., Moscou, Matthew J., Liang, Yong, Yu, Guotai, Wulff, Brande B. H., Muehlbauer, Gary, Minz-Dub, Anna and Sharon, Amir (2024) A single NLR gene confers resistance to leaf and stripe rust in wheat. Nature Communications, 15 (1). ISSN 2041-1723

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Abstract

Nucleotide-binding leucine-rich repeat (NLR) disease resistance genes typically confer resistance against races of a single pathogen. Here, we report that Yr87/Lr85, an NLR gene from Aegilops sharonensis and Aegilops longissima, confers resistance against both P. striiformis tritici (Pst) and Puccinia triticina (Pt) that cause stripe and leaf rust, respectively. Yr87/Lr85 confers resistance against Pst and Pt in wheat introgression as well as transgenic lines. Comparative analysis of Yr87/Lr85 and the cloned Triticeae NLR disease resistance genes shows that Yr87/Lr85 contains two distinct LRR domains and that the gene is only found in Ae. sharonensis and Ae. longissima. Allele mining and phylogenetic analysis indicate multiple events of Yr87/Lr85 gene flow between the two species and presence/absence variation explaining the majority of resistance to wheat leaf rust in both species. The confinement of Yr87/Lr85 to Ae. sharonensis and Ae. longissima and the resistance in wheat against Pst and Pt highlight the potential of these species as valuable sources of disease resistance genes for wheat improvement.

Item Type:	Article
Additional Information:	Funding Information: The research was supported by BARD grant # IS-5087-18 R to A.M-D., A.S., and G.M., The Lieberman Family and the JNF Australia donations to A.S., and by the Lieberman-Okinow Endowment at Tel Aviv University to the Institute for Cereal Crops Research. D.S. was supported by PBC fellowship of the Council for Higher Education of Israel; M.J.M. received funding from the 2Blades Foundation, Japan Tobacco Inc., KANEKA CORPORATION, the United Kingdom Research and Innovation-Biotechnology and Biological Sciences Research Council Institute Strategic Programme (UKRI-BBSRC ISP; grant no. BBS/E/J/000PR9795), the Gatsby Charitable Foundation, and United States Department of Agriculture-Agricultural Research Service CRIS #5062-21220-025-000D; BBHW received funding from the 2Blades Foundation, and the UKRI-BBSRC ISP (grant no. BBS/E/J/000PR9780); J.D. received funding from ERDF project \u2018Plants as a tool for sustainable global development\u2019, No. CZ.02.1.01/0.0/0.0/16_019/0000827. We thank Zde\u0148ka Dubsk\u00E1, Romana \u0160perkov\u00E1, and Jitka Weiserov\u00E1 for the preparation of chromosome samples for flow cytometry. We thank Cathy Melamed-Bessudo for providing the modified CTAB protocol (PacBio-grade sequencing) and Yamit Bar-Lev for assistance with the DNA extraction process. Publisher Copyright: © The Author(s) 2024.
Uncontrolled Keywords:	chemistry(all),biochemistry, genetics and molecular biology(all),physics and astronomy(all) ,/dk/atira/pure/subjectarea/asjc/1600
Faculty \ School:	Faculty of Science > The Sainsbury Laboratory
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	14 Jan 2025 00:59
Last Modified:	28 Jan 2025 23:48
URI:	https://ueaeprints.uea.ac.uk/id/eprint/98195
DOI:

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