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ToolsWang, Shu-Yan, Zhang, Nan, Teng, Zhao-Jie, Wang, Xiao-Di, Todd, Jonathan D., Zhang, Yu-Zhong, Cao, Hai-Yan and Li, Chun-Yang (2023) A new dimethylsulfoniopropionate lyase of the cupin superfamily in marine bacteria. Environmental Microbiology, 25 (7). pp. 1238-1249. ISSN 1462-2912
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Abstract
Dimethylsulfoniopropionate (DMSP) is a marine organosulfur compound with important roles in stress protection, marine biogeochemical cycling, chemical signalling and atmospheric chemistry. Diverse marine microorganisms catabolize DMSP via DMSP lyases to generate the climate-cooling gas and info-chemical dimethyl sulphide. Abundant marine heterotrophs of the Roseobacter group (MRG) are well known for their ability to catabolize DMSP via diverse DMSP lyases. Here, a new DMSP lyase DddU within the MRG strain Amylibacter cionae H-12 and other related bacteria was identified. DddU is a cupin superfamily DMSP lyase like DddL, DddQ, DddW, DddK and DddY, but shares <15% amino acid sequence identity with these enzymes. Moreover, DddU proteins forms a distinct clade from these other cupin-containing DMSP lyases. Structural prediction and mutational analyses suggested that a conserved tyrosine residue is the key catalytic amino acid residue in DddU. Bioinformatic analysis indicated that the dddU gene, mainly from Alphaproteobacteria, is widely distributed in the Atlantic, Pacific, Indian and polar oceans. For reference, dddU is less abundant than dddP, dddQ and dddK, but much more frequent than dddW, dddY and dddL in marine environments. This study broadens our knowledge on the diversity of DMSP lyases, and enhances our understanding of marine DMSP biotransformation.
| Item Type: | Article |
|---|---|
| Additional Information: | Data Availability Statement: The data that support the findings of this study are available in the figures, table and supporting information of this article. Funding Information: This work was supported by the National Key Research and Development Program of China (2022YFC2807500, 2021YFA0909600), the National Science Foundation of China (grants 92251303, 42276102, 42076229, 91851205, 31961133016, 42206156), the Fundamental Research Funds for the Central Universities (202172002, 202041011), the Major Scientific and Technological Innovation Project (MSTIP) of Shandong Province (2019JZZY010817), the Program of Shandong for Taishan Scholars (tspd20181203), the Biotechnology and Biological Sciences Research Council, UK, grant (BB/X005968) and Natural Environment Research Council, UK, Standard grants (NE/X000990, NE/V000756 and NE/S001352). |
| Uncontrolled Keywords: | microbiology,ecology, evolution, behavior and systematics,sdg 13 - climate action,sdg 14 - life below water ,/dk/atira/pure/subjectarea/asjc/2400/2404 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Molecular Microbiology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 14 Jan 2025 00:59 |
| Last Modified: | 20 Jan 2025 01:07 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/98194 |
| DOI: | 10.1111/1462-2920.16355 |
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