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Mishra, Prashastha, Barrera, Tania S., Grieshop, Karl 
ORCID: https://orcid.org/0000-0001-8925-5066 and Agrawal, Aneil F.
(2024)
Cis-regulatory variation in relation to sex and sexual dimorphism in Drosophila melanogaster.
Genome Biology and Evolution, 16 (11).
ISSN 1759-6653
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Abstract
Much of sexual dimorphism is likely due to sex-biased gene expression, which results from differential regulation of a genome that is largely shared between males and females. Here, we use allele-specific expression to explore cis-regulatory variation in Drosophila melanogaster in relation to sex. We develop a Bayesian framework to infer the transcriptome-wide joint distribution of cis-regulatory effects across the sexes. We also examine patterns of cis-regulatory variation with respect to two other levels of variation in sexual dimorphism: (i) across genes that vary in their degree of sex-biased expression and (ii) among tissues that vary in their degree of dimorphism (e.g. relatively low dimorphism in heads vs. high dimorphism in gonads). We uncover evidence of widespread cis-regulatory variation in all tissues examined, with female-biased genes being especially enriched for this variation. A sizeable proportion of cis-regulatory variation is inferred to have sex-specific effects, with sex-dependent cis effects being much more frequent in gonads than in heads. Finally, we find some genes where 1 allele contributes to more than 50% of a gene's expression in heterozygous males but <50% of its expression in heterozygous females. Such variants could provide a mechanism for sex-specific dominance reversals, a phenomenon important for sexually antagonistic balancing selection. However, tissue differences in allelic imbalance are approximately as frequent as sex differences, perhaps suggesting that sexual conflict may not be particularly unique in shaping patterns of expression variation.
| Item Type: | Article |
|---|---|
| Additional Information: | Data Availability Statement: RNA-seq data are available at the NIH Sequence Read Archive (accession: PRJNA1173240) and analysis scripts at https://github.com/mishrap1971/Mishra_et_al_Drosophila_ASE. Funding Information: This work was supported by the Natural Sciences and Engineering Research Council of Canada (Discovery Grant to A.F.A.). |
| Uncontrolled Keywords: | allelic imbalance,sex-biased gene expression,sexual dimorphism,medicine(all) ,/dk/atira/pure/subjectarea/asjc/2700 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 14 Jan 2025 00:59 |
| Last Modified: | 14 Jan 2025 00:59 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/98190 |
| DOI: | 10.1093/gbe/evae234 |
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