The zebrafish sperm proteome

Cohen-Krais, Jayme, Martins, Carlo, Bartram, Jamie, Crighton, Zoe, de Coriolis, Jean-Charles, Godden, Alice ORCID: https://orcid.org/0000-0001-5760-011X, Marcu, Daniel ORCID: https://orcid.org/0000-0002-2151-5850, Robak, Weronika, Saalbach, Gerhard and Immler, Simone (2024) The zebrafish sperm proteome. Proteomics. ISSN 1615-9853

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Abstract

One of the key processes that forms the basis of fertilisation is the tight interaction between sperm and egg. Both sperm and egg proteomes are known to evolve and diverge rapidly even between closely related species. Understanding the sperm proteome therefore provides key insights into the proteins that underpin the mechanisms involved during fertilisation and the fusion between sperm and egg, and how they can differ across individuals of the same species. Despite being a commonly used model organism for reproductive research, little is currently understood about the zebrafish Danio rerio sperm proteome. We performed nanoLC-MS/MS proteomics analysis after off-line sample fractionation with six pooled samples containing sperm from ten males each. We confidently identified 5410 proteins, from which a total of 3900 GeneIDs were generated leading to 1720 Gene Ontology terms.

Item Type:	Article
Additional Information:	Data Availability Statement: The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD056057 and https://doi.org/10.6019/PXD056057. Funding information: This study was funded by grants from the Natural Environment Research Council (NE/S011188/1) and the European Research Council (SELECTHAPLOID-101001341) to SI.
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Science
UEA Research Groups:	Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation
Related URLs:	https://analyticalsciencejournals.online...
Depositing User:	LivePure Connector
Date Deposited:	10 Jan 2025 01:00
Last Modified:	13 Jan 2025 01:01
URI:	https://ueaeprints.uea.ac.uk/id/eprint/98143
DOI:	10.1002/pmic.202400310

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