Assessing the potential of a genetically modified Parachlorella kessleri-I with low CO2 inducible proteins for enhanced biomass and biofuel productivity

Singh, Amit Kumar, Nawkarkar, Prachi, Bhatnagar, Vipul Swarup, Tripathi, Shweta, Mock, Thomas ORCID: https://orcid.org/0000-0001-9604-0362 and Kumar, Shashi (2024) Assessing the potential of a genetically modified Parachlorella kessleri-I with low CO2 inducible proteins for enhanced biomass and biofuel productivity. Journal of Environmental Chemical Engineering, 12 (5). ISSN 2213-3437

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Abstract

The biological process is one of the promising approaches for CO2 capture and storage. Recently, biological sequestration using microalgae has gained much interest due to its capability to utilize CO2 as a carbon source to generate valorizable biomass. This algal biomass further can be used as a feedstock for bioenergy and different value-added products. Here, we have heterogeneously overexpressed the low CO2 inducible proteins (LCIA & LCIB) from Chlamydomonas reinhardtii in the marine alga Parachlorella kessleri-I. Incorporation of these two genes boosted the endogenous CCM leading to a hybrid system and improved overall carbon acquisition. The combination of these CCM proteins enhanced the supply of inorganic carbon to RuBisCO with positive effects on growth and biomass productivity. Biochemical analyses revealed that the hybrid CCM in P. kessleri-I was characterised by 2- fold higher carbonic anhydrase activity, increased starch, and 2X more lipid yield in comparison to wild type (WT) strain. Moreover, the semi-continuous cultivation of GM strain with supply of 1 % CO2 led to around 60 % rise in the overall biomass productivity. These traits also persisted in scale-up studies leading to 2X more biomass productivity in the GM strain than WT in 100 L PBR. A comparative life-cycle assessment underlines the sustainability of the process of carbon capture and both biofuel and biochar production from P. kessleri-I with a hybrid CCM.

Item Type: Article
Additional Information: Funding Information: Funding from Department of Biotechnology (DBT), Government of India, supported this work. Publisher Copyright: © 2024 Elsevier Ltd
Uncontrolled Keywords: biofuel,carbon capture,co-concentrating mechanism,inorganic carbon,microalgal biomass,chemical engineering (miscellaneous),waste management and disposal,pollution,process chemistry and technology,sdg 7 - affordable and clean energy,sdg 12 - responsible consumption and production,sdg 14 - life below water ,/dk/atira/pure/subjectarea/asjc/1500/1501
Faculty \ School: University of East Anglia Research Groups/Centres > Theme - ClimateUEA
Faculty of Science > School of Environmental Sciences
UEA Research Groups: Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation
Faculty of Science > Research Groups > Environmental Biology
Faculty of Science > Research Groups > Centre for Ocean and Atmospheric Sciences
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Depositing User: LivePure Connector
Date Deposited: 04 Dec 2024 01:41
Last Modified: 09 Dec 2024 01:38
URI: https://ueaeprints.uea.ac.uk/id/eprint/97891
DOI: 10.1016/j.jece.2024.113795

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