Phytophthora sojae boosts host trehalose accumulation to acquire carbon and initiate infection

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Zhu, Xiaoguo, Fang, Di, Li, Die, Zhang, Jianing, Jiang, Haixin, Guo, Liang, He, Qingyuan, Zhang, Tianyu, Macho, Alberto P., Wang, Ertao, Shen, Qian-Hua, Wang, Yuanchao, Zhou, Jian-Min, Ma, Wenbo and Qiao, Yongli (2023) Phytophthora sojae boosts host trehalose accumulation to acquire carbon and initiate infection. Nature Microbiology, 8. pp. 1561-1573. ISSN 2058-5276

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Abstract

Successful infection by pathogenic microbes requires effective acquisition of nutrients from their hosts. Root and stem rot caused by Phytophthora sojae is one of the most important diseases of soybean (Glycine max). However, the specific form and regulatory mechanisms of carbon acquired by P. sojae during infection remain unknown. In the present study, we show that P. sojae boosts trehalose biosynthesis in soybean through the virulence activity of an effector PsAvh413. PsAvh413 interacts with soybean trehalose-6-phosphate synthase 6 (GmTPS6) and increases its enzymatic activity to promote trehalose accumulation. P. sojae directly acquires trehalose from the host and exploits it as a carbon source to support primary infection and development in plant tissue. Importantly, GmTPS6 overexpression promoted P. sojae infection, whereas its knockdown inhibited the disease, suggesting that trehalose biosynthesis is a susceptibility factor that can be engineered to manage root and stem rot in soybean.

Item Type:	Article
Additional Information:	Funding Information: We thank Y. Wang (Nanjing Agricultural University) for providing the P. Sojae isolate P6497-RFP, H. Zhang (Shanghai Normal University, China) for soybean CRISPR–Cas9 vector and S. Dong (Nanjing Agricultural University) for helpful discussions. This work was supported by grants from the National Natural Science Foundation of China (grant nos. 32001883 and 32072502) and the Science and Technology Commission of Shanghai Municipality (grant no. 18DZ2260500). Funding Information: We thank Y. Wang (Nanjing Agricultural University) for providing the P. Sojae isolate P6497-RFP, H. Zhang (Shanghai Normal University, China) for soybean CRISPR–Cas9 vector and S. Dong (Nanjing Agricultural University) for helpful discussions. This work was supported by grants from the National Natural Science Foundation of China (grant nos. 32001883 and 32072502) and the Science and Technology Commission of Shanghai Municipality (grant no. 18DZ2260500). Publisher Copyright: © 2023, The Author(s), under exclusive licence to Springer Nature Limited.
Uncontrolled Keywords:	microbiology,immunology,applied microbiology and biotechnology,genetics,microbiology (medical),cell biology ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School:	Faculty of Science > The Sainsbury Laboratory
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	03 Dec 2024 01:35
Last Modified:	03 Dec 2024 01:35
URI:	https://ueaeprints.uea.ac.uk/id/eprint/97863
DOI:	10.1038/s41564-023-01420-z

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