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ToolsRunde, Joseph, Veseli, Iva, Fogarty, Emily C., Watson, Andrea R., Clayssen, Quentin, Yosef, Mahmoud, Shaiber, Alon, Verma, Ritu, Quince, Christopher, Gerasimidis, Konstantinos, Rubin, David T. and Eren, A. Murat (2023) Transient suppression of bacterial populations associated with gut health is critical in success of exclusive enteral nutrition for children with Crohn's disease. Journal of Crohn's and Colitis, 17 (7). pp. 1103-1113. ISSN 1873-9946
Full text not available from this repository. (Request a copy)Abstract
Background and Aims: Exclusive enteral nutrition [EEN] is a dietary intervention to induce clinical remission in children with active luminal Crohn's disease [CD]. While changes in the gut microbial communities have been implicated in achieving this remission, a precise understanding of the role of microbial ecology in the restoration of gut homeostasis is lacking. Methods: Here we reconstructed genomes from the gut metagenomes of 12 paediatric subjects who were sampled before, during and after EEN. We then classified each microbial population into distinct 'phenotypes' or patterns of response based on changes in their relative abundances throughout the therapy on a per-individual basis. Results: Our data show that children achieving clinical remission during therapy were enriched with microbial populations that were either suppressed or that demonstrated a transient bloom as a function of EEN. In contrast, this ecosystem-level response was not observed in cases of EEN failure. Further analysis revealed that populations that were suppressed during EEN were significantly more prevalent in healthy children and adults across the globe compared with those that bloomed ephemerally during the therapy. Conclusions: These observations taken together suggest that successful outcomes of EEN are marked by a temporary emergence of microbial populations that are rare in healthy individuals, and a concomitant reduction in microbes that are commonly associated with gut homeostasis. Our work is a first attempt to highlight individual-specific, complex environmental factors that influence microbial response in EEN. This model offers a novel, alternative viewpoint to traditional taxonomic strategies used to characterize associations with health and disease states.
| Item Type: | Article |
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| Additional Information: | Funding Information: We thank the members of the department of Pediatrics, section of Gastroenterology, Hepatology and Nutrition at the University of Chicago Medical Center for support and guidance at the inception of this study. Additionally, we thank the department of Pediatrics, section of Gastroenterology, Hepatology and Nutrition at the Ann & Robert H. Lurie Children’s Hospital of Chicago for supporting this research effort. IV acknowledges support by the National Science Foundation Graduate Research Fellowship, under Grant No. 1746045. Publisher Copyright: © 2023 The Author(s). Published by Oxford University Press on behalf of European Crohn's and Colitis Organisation. All rights reserved. |
| Uncontrolled Keywords: | genome-resolved metagenomics,gut microbiome,microbial therapeutics,medicine(all) ,/dk/atira/pure/subjectarea/asjc/2700 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 03 Dec 2024 01:35 |
| Last Modified: | 03 Dec 2024 01:35 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97860 |
| DOI: | 10.1093/ecco-jcc/jjad031 |
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