The host exocyst complex is targeted by a conserved bacterial type-III effector that promotes virulence

Michalopoulou, Vassiliki A., Mermigka, Glykeria, Kotsaridis, Konstantinos, Mentzelopoulou, Andriani, Celie, Patrick H. N., Moschou, Panagiotis N., Jones, Jonathan D. G. and Sarris, Panagiotis F. (2022) The host exocyst complex is targeted by a conserved bacterial type-III effector that promotes virulence. Plant Cell, 34 (9). pp. 3400-3424. ISSN 1040-4651

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Abstract

For most Gram-negative bacteria, pathogenicity largely depends on the type-III secretion system that delivers virulence effectors into eukaryotic host cells. The subcellular targets for the majority of these effectors remain unknown. Xanthomonas campestris, the causal agent of black rot disease of crucifers such as Brassica spp., radish, and turnip, delivers XopP, a highly conserved core-effector protein produced by X. campestris, which is essential for virulence. Here, we show that XopP inhibits the function of the host–plant exocyst complex by direct targeting of Exo70B, a subunit of the exocyst complex, which plays a significant role in plant immunity. XopP interferes with exocyst-dependent exocytosis and can do this without activating a plant NOD-like receptor that guards Exo70B in Arabidopsis. In this way, Xanthomonas efficiently inhibits the host’s pathogen-associated molecular pattern (PAMP)-triggered immunity by blocking exocytosis of pathogenesis-related protein-1A, callose deposition, and localization of the FLAGELLIN SENSITIVE2 (FLS2) immune receptor to the plasma membrane, thus promoting successful infection. Inhibition of exocyst function without activating the related defenses represents an effective virulence strategy, indicating the ability of pathogens to adapt to host defenses by avoiding host immunity responses.

Item Type:	Article
Additional Information:	Funding Information: We especially want to thank Prof. Marco Trujillo for his generous donation of the AtEXO70B2 construct as well as for his advice on construction of the truncations construction; Prof. M. Moscou for his kind donation of some of the IDs used in this research; Dr. Katarzyna Rybak for the kind donation of the FLS2–GFP construct; Prof. František Baluška for his kind donation of the DsRed-FYVE confocal marker; Dr. Yasin Dagdas for his generous donation of all the other confocal markers used in the research, and for fruitful discussion; Mr Marc Youles and the Synthetic Biology Group of TSL for the kind donation of Golden-Gate compatible Y2H vectors; Prof. Nemo Peeters for his kind donation of Rs GMI1000; Prof. Dimitris Goumas for his generous donation of Xcv; Prof. Subba Rao Gangi Setty for his kind donation of the pBridge vector; Dr. Jeff Chang for his kind donation of Pf01 EtHAn; Prof. D. Alexandraki for her kind donation of PJ69 yeast strain and her guidance with Y2H experiments; and lastly undergraduate student Aspa Papanikolaou for her kind contribution in some of the Y2H assays. V.A.M. was supported by the Hellenic Foundation for Research and Innovation (HFRI) and the General Secretariat for Research and Technology (GSRT), under the HFRI PhD Fellowship grant (GA. no. 4776) and by the internal PhD supporting scheme of IMBB-FORTH. K.K. and P.F.S. were supported by the European Union and Greek national funds through the Operational Program Competitiveness, Entrepreneurship and Innovation, under the call RESEARCH–CREATE–INNOVATE (“INNOVA-PROTECT” with project code: T1EDK-01878). P.N.M and A.M. were supported by the FORMAS Research Council Grant MOP-86675, and HFRI grants Always Strive for Excellence 1624 and Ph.D. scholarship. P.H.N.C.’s work benefited from access to the NKI Protein Facility and the Instruct-ERIC center, and was supported by iNEXT-Discovery, project number 871037, funded by the Horizon 2020 program of the European Commission Publisher Copyright: © American Society of Plant Biologists 2022. All rights reserved.
Uncontrolled Keywords:	plant science ,/dk/atira/pure/subjectarea/asjc/1100/1110
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Plant Sciences
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	03 Dec 2024 01:35
Last Modified:	03 Dec 2024 01:35
URI:	https://ueaeprints.uea.ac.uk/id/eprint/97858
DOI:	10.1093/plcell/koac162

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