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ToolsNirwan, Shradha, Chatterjee, Anupriya, Cevik, Volkan, Holub, Eric B., Jones, Jonathan D. G., Tewari, Anand Kumar, Shrivastava, Neeraj, Agnihotri, Abha and Sharma, Pankaj (2024) Genetic manipulation of Indian mustard genotypes with WRR-gene(s) confers resistance against Albugo candida. Molecular Biology Reports, 51. ISSN 0301-4851
Full text not available from this repository. (Request a copy)Abstract
Background: Brassica species is the second most important edible oilseed crop in India. Albugo candida (Pers.) Kuntze, a major oomycete disease of oilseed brassica causing white rust, leads to 60% yield loss globally. The prevalence of A. candida race 2 (Ac2V) that specifically infects B. juncea, coupled with limitations of conventional methods has resulted in a dearth of white rust resistance resources in cultivated varieties. Methods and results: In an effort to develop resistant plants, Agrobacterium mediated genetic transformation of three B. juncea genotypes viz., susceptible host var. Varuna, along with its doubled haploid mutant lines C66 and C69 (showing moderate tolerance to field isolates of A. candida) was initiated to transfer resistance genes (WRR8Sf-2 and WRR9Hi-0) identified in Arabidopsis thaliana against race Ac2V, that encode for Toll-like/interleukin-1 receptor-nucleotide binding-leucine-rich repeat proteins that recognize effectors of the pathogen races. Conclusions: Our results demonstrate that introduction of resistance genes from a tertiary gene pool by genetic transformation enhances disease resistance in B. juncea genotypes to a highly virulent Ac2V isolate.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Information: We are grateful to Prof. Deepak Pental and Prof. MV Rajam, University of Delhi South Campus, New Delhi and their associates for help in genetic transformation and molecular work. Funding Information: Authors are thankful to the India Department of Biotechnology (DBT) and the UK Biotechnology and Biological Sciences Research Council (BBSRC) for joint funding of this collaborative work as a Crop Genomics and Technologies (CGAT) project: DBT funding BT/IN/Indo-UK/CGAT/12/DP/2014-2015 & BBSRC funding BB/L011646/1. Publisher Copyright: © 2024, The Author(s), under exclusive licence to Springer Nature B.V. |
| Uncontrolled Keywords: | agrobacterium-mediated transformation,albugo candida,brassica juncea,white rust,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/1300/1312 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Plant Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 03 Dec 2024 01:35 |
| Last Modified: | 03 Dec 2024 01:35 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/97855 |
| DOI: | 10.1007/s11033-023-09040-w |
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